Core Facilities

Flow Cytometry

Overview
Staff
Equipment
Contact
  • flow-labThe Flow Cytometry Core Facility (FCCF), is housed in the Terry Fox Laboratory and provides investigators at the British Columbia Cancer Research Centre access to high quality, cost effective flow cytometry services. By providing these services, as well as the scientific expertise necessary to effectively use this technology, the facility serves to enhance the scope and quality of scientific research performed at the centre.

  • Wenbo Xu

    Cancer Research Technologist
  • BD FACSAria II
    FACSAria-sm
    A high-speed cell-sorting instrument equipped with 405nm, 488nm and 640nm lasers. Detector combination: FSC, SSC, 5 blue, 2 red, and 2 violet.
    BD FACSAria III
    A biosafety cabinet-enclosed high-speed cell-sorting instrument equipped with 375nm, 405nm, 488nm, 561nm and 640nm laser. Detector combination: 1) FSC, SSC, 2 blue, 5 yellow-green, 6 violet, and 3 red. 2) FSC, SSC, 2 blue, 5 yellow-green, 2 UV, and 3 red
    BD FACSAria Fusion
    A biosafety cabinet-enclosed high-speed cell sorting instrument equipped with 375nm, 405nm, 445nm, 488nm, 561nm, and 640nm lasers. Detector combination: 1) FSC, SSC, 2 blue or 445nm, 5 yellow-green, 6 violet, and 3 red. 2) FSC, SSC, 2 blue or 445nm, 5 yellow-green, 2 UV, and 3 red
    BD Influx I
    A cell sorting instrument equipped with 561nm, 640, and 2 tunable lasers. Detector combination: 1) FSC, SSC, 5 blue, 2 red, and 2 UV. 2) FSC, SSC, 5 blue, 2 yellow-green, and 2 UV
    BD Influx II
    A high-speed cell sorting instrument equipped with 405nm, 488nm and 640nm lasers. Detector combination: FSC, SSC, 5 blue, 2 red, and 2 violet.
    BD Fortessa I
    FortessaI-II-sm
    A multicolour analyzer with 405nm, 488nm, 561nm and 640nm lasers. Detector combination: 2 blue, 5 yellow-green, 6 violet, and 3 red
    BD Fortessa II
    FortessaI-II-sm A multicolour analyzer with 405nm, 488nm, 561nm and 640nm lasers. Detector combination: 2 blue, 5 yellow-green, 6 violet, and 3 red
    Fluidigm DVS CyTOF 2
    CyTOF-smA state-of-the-art instrument that is the combination of Flow Cytometry and Mass Spectrometry that allows over 40 biomarker detection simultaneously.
    BD FACSCalibur - Tangerine
    An analyzer with 488nm and 640nm laser. Colour combination: FSC, SSC, 3 blue, and 1 red
    BD FACSCalibur - Pistachio
    An analyzer with 488nm and 640nm laser. Colour combination: FSC, SSC, 3 blue, and 1 red
    VetNovations VetABC blood analyzer

    An automated hematology analyzer capable of analyzing blood samples from various animal species.

    Parameters detected:

    WBC, RBC, PLT, HGB, HCT, MCV, MCH, MCHC, RDW, MPV
    Lymphocytes (% and #), Monocytes (% and #), Granulocytes (% and #), Eosinophiles (%)
    3 Histograms – Leukocytes, Erythrocytes & Thrombocytes

    Beckman Coulter vi-CELL viability analyzer

    Provides a cost effective means to perform the trypan blue dye exclusion method. Allows users to load up to 10 samples at once for easy and automated cell counting.
     

    Product Features:

    • % Viability
    • Total cell concentration
    • Total viable cell concentration
    • Mean cell size
    • Real time cellular images
  • Flow Cytometry Core Facility

    BC Cancer Research Center
    675 West 10th Avenue, Rm 11-218
    Vancouver, BC

    Email:

    Tel: 604-675-8000 ext 7738/7737

    Fax: 604-877-0712