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Methylated DNA immunoprecipitation analysis of mammalian endogenous retroviruses.
Rebollo R & Mager DL. Methylated DNA immunoprecipitation analysis of mammalian endogenous retroviruses. Methods Mol Biol 1400: 377-385, 2016.
Endogenous retroviruses are repetitive sequences found abundantly in mammalian genomes which are capable of modulating host gene expression. Nevertheless, most endogenous retrovirus copies are under tight epigenetic control via histone-repressive modifications and DNA methylation. Here we describe a common method used in our laboratory to detect, quantify, and compare mammalian endogenous retrovirus DNA methylation. More specifically we describe methylated DNA immunoprecipitation (MeDIP) followed by quantitative PCR.