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Dr Allen C Eaves — MD, PhD, FRCP(C)

Distinguished Scientist

Overview
Research Interest
Publications
  • Professor Emeritus, Medicine, UBC
  • Professor Emeritus, Pathology & Laboratory Medicine, UBC
  • Leukemia and the Myeloproliferative and Myelodysplastic Syndromes
  • Bone Marrow Transplantation

My research objectives are to develop in vitro and animal model systems to allow key biological and molecular features of human leukemic hematopoiesis to be identified and reproduced, and to allow assessment of the effects of manipulating leukemic stem cell populations under defined conditions. At present, our efforts are focused on chronic myeloid leukemia, a disease with an annual incidence of 1 per 100,000 and curable in only a limited number of cases, primarily with high dose therapy and a sibling bone marrow transplant.

Chronic myeloid leukemia (CML) arises in a pluripotent hematopoietic stem cell as a result of a specific rearrangement of the BCR gene on chromosome 22 and the c-ABL protooncogene on chromosome 9. This rearrangement results in the formation of the Ph chromosome and a novel BCR-ABL fusion gene which encodes a protein with oncogenic properties. Although the specific molecular pathways by which BCR-ABL mediates its biological effects to cause CML remain largely unknown, a specific inhibitor of the BCR-ABL kinase is now showing great clinical promise and provides a unique experimental tool to test how this gene alters stem cell function.

We have recently developed methods for purifying both leukemic and residual normal stem cells from patients with CML. This has allowed us to analyze their biological and molecular properties and to compare them to BCR-ABL-transduced primary human and murine hematopoietic cells. An early outcome of this effort has been our discovery that the BCR-ABL oncogene activates an autocrine growth mechanism selectively in primitive hematopoietic cells. More recently we have found that this oncogene can also cause hematopoietic lineage switching when it is overexpressed in transduced hematopoietic cells.

Sloma I, Jiang X, Eaves AC, Eaves CJ.  Insights into the stem cells of chronic myeloid leukemia. Leukemia  24(11):1823-1833, 2010. View Abstract

Jiang X, Forrest D, Nicolini F, Turhan A, Guilhot J, Yip C, Holyoake T, Jorgensen H, Lambie K, Saw KM, Pang E, Vukovic R, Lehn P, Ringrose A, Yu M, Brinkman RR, Smith C, Eaves A and Eaves C. Properties of CD34+ CML stem/progenitor cells that correlate with clinical response to imatinib mesylate. Blood 116: 2112-2121, 2010. View Abstract

Vercauteren SM, Bashashati A, Wu D, Brinkman RR, Eaves C, Eaves A, & Karsan A. Reduction in multi-lineage and erythroid progenitors distinguishes myelodysplastic syndromes from non-malignant cytopenias. Leuk Res 33 (12): 1636-42, 2009. View Abstract

Starczynowski DT, Vercauteren S, Telenius A, Sung S, Tohyama K, Brooks-Wilson A, Spinelli JJ, Eaves CJ, Eaves AC, Horsman DE, Lam WL, & Karsan A. High-resolution whole genome tiling path array CGH analysis of CD34+ cells from patients with low-risk myelodysplastic syndromes reveals cryptic copy number alterations and predicts overall and leukemia-free survival. Blood 112 (8): 3412-24, 2008. View Abstract

Jiang X, Zhao Y, Forrest D, Smith C, Eaves A, & Eaves C. Stem cell biomarkers in chronic myeloid leukemia. Dis Markers 24 (4-5): 201-16, 2008. View Abstract

Louis SA, Rietze RL, Deleyrolle L, Wagey RE, Thomas TE, Eaves AC, & Reynolds BA. Enumeration of Neural Stem and Progenitor Cells in the Neural Colony Forming Cell Assay. Stem Cells, 2008. View Abstract

Clarke E, Pereira C, Chaney R, Woodside S, Eaves AC, & Damen J. Toxicity testing using hematopoietic stem cell assays. Regen Med 2 (6): 947-56, 2007. View Abstract

Christ O, Lucke K, Imren S, Leung K, Hamilton M, Eaves A, Smith C, & Eaves C. Improved purification of hematopoietic stem cells based on their elevated aldehyde dehydrogenase activity. Haematologica 92 (9): 1165-72, 2007. View Abstract

Jiang X, Saw KM, Eaves A, & Eaves C. Instability of BCR-ABL gene in primary and cultured chronic myeloid leukemia stem cells. J Natl Cancer Inst 99 (9): 680-93, 2007. View Abstract

Jiang X, Smith C, Eaves A, & Eaves C. The challenges of targeting chronic myeloid leukemia stem cells. Clin Lymphoma Myeloma 7 Suppl 2: S71-80, 2007. View Abstract

Jiang X, Zhao Y, Smith C, Gasparetto M, Turhan A, Eaves A, & Eaves C. Chronic myeloid leukemia stem cells possess multiple unique features of resistance to BCR-ABL targeted therapies. Leukemia 21 (5): 926-35, 2007. View Abstract

Yang H, Eaves C, de Lima M, Lee MS, Champlin RE, McMannis JD, Robinson SN, Niu T, Decker WK, Xing D, Ng J, Li S, Yao X, Eaves AC, Jones R, Andersson BS, & Shpall EJ. A novel triple purge strategy for eliminating chronic myelogenous leukemia (CML) cells from autografts. Bone Marrow Transplant 37 (6): 575-82, 2006. View Abstract

Jorgensen HG, Copland M, Allan EK, Jiang X, Eaves A, Eaves C, & Holyoake TL. Intermittent exposure of primitive quiescent chronic myeloid leukemia cells to granulocyte-colony stimulating factor in vitro promotes their elimination by imatinib mesylate. Clin Cancer Res 12 (2): 626-33, 2006. View Abstract

Peters CE, Woodside SM, & Eaves AC. Isolation of subsets of immune cells. Methods Mol Biol 302: 95-116, 2005. View Abstract

Eisterer W, Jiang X, Christ O, Glimm H, Lee KH, Pang E, Lambie K, Shaw G, Holyoake TL, Petzer AL, Auewarakul C, Barnett MJ, Eaves CJ, & Eaves AC. Different subsets of primary chronic myeloid leukemia stem cells engraft immunodeficient mice and produce a model of the human disease. Leukemia 19 (3): 435-41, 2005. View Abstract

Chalandon Y, Jiang X, Christ O, Loutet S, Thanopoulou E, Eaves A, & Eaves C. BCR-ABL-transduced human cord blood cells produce abnormal populations in immunodeficient mice. Leukemia 19 (3): 442-8, 2005. View Abstract

Chalandon Y, Jiang X, Loutet S, Eaves AC, & Eaves CJ. Growth autonomy and lineage switching in BCR-ABL-transduced human cord blood cells depend on different functional domains of BCR-ABL. Leukemia 18 (5): 1006-12, 2004. View Abstract

Thanopoulou E, Cashman J, Kakagianne T, Eaves A, Zoumbos N, & Eaves C. Engraftment of NOD/SCID-beta2 microglobulin null mice with multilineage neoplastic cells from patients with myelodysplastic syndrome. Blood 103 (11): 4285-93, 2004. View Abstract

Jiang X, Zhao Y, Chan WY, Vercauteren S, Pang E, Kennedy S, Nicolini F, Eaves A, & Eaves C. Deregulated expression in Ph+ human leukemias of AHI-1, a gene activated by insertional mutagenesis in mouse models of leukemia. Blood 103 (10): 3897-904, 2004. View Abstract

Wognum AW, Eaves AC, & Thomas TE. Identification and isolation of hematopoietic stem cells. Arch Med Res 34 (6): 461-75, 2003. View Abstract

Eaves A, & Palmer AR. Reproduction: widespread cloning in echinoderm larvae. Nature 425 (6954): 146, 2003. View Abstract

Jorgensen HG, Allan EK, Jiang X, Liakopoulou E, Richmond L, Eaves CJ, Eaves AC, & Holyoake TL. Stage-specific alterations in serum levels of G-CSF in chronic myeloid leukaemia. Leukemia 17 (7): 1430-2, 2003. View Abstract

Jiang X, Stuible M, Chalandon Y, Li A, Chan WY, Eisterer W, Krystal G, Eaves A, & Eaves C. Evidence for a positive role of SHIP in the BCR-ABL-mediated transformation of primitive murine hematopoietic cells and in human chronic myeloid leukemia. Blood 102 (8): 2976-84, 2003. View Abstract

Eaves C, Jiang X, Eisterer W, Chalandon Y, Porada G, Zanjani E, & Eaves A. New models to investigate mechanisms of disease genesis from primitive BCR-ABL(+) hematopoietic cells. Ann N Y Acad Sci 996: 1-9, 2003. View Abstract

Scanlon L, & Eaves A. Pressure relieving equipment selection in the community: rising to the challenge. Br J Community Nurs: 19-24, 2002. View Abstract

Richmond LJ, Alcorn MJ, Pearson C, Cameron G, Thomas T, Eaves CJ, Eaves AC, & Holyoake TL. CML leukapheresis products can be enriched for CD34+ cells and simultaneously depleted of CD15+ cells using a simple Ab cocktail. Cytotherapy 4 (5): 407-13, 2002. View Abstract

Cashman J, Dykstra B, Clark-Lewis I, Eaves A, & Eaves C. Changes in the proliferative activity of human hematopoietic stem cells in NOD/SCID mice and enhancement of their transplantability after in vivo treatment with cell cycle inhibitors. J Exp Med 196 (9): 1141-9, 2002. View Abstract

Jiang X, Ng E, Yip C, Eisterer W, Chalandon Y, Stuible M, Eaves A, & Eaves CJ. Primitive interleukin 3 null hematopoietic cells transduced with BCR-ABL show accelerated loss after culture of factor-independence in vitro and leukemogenic activity in vivo. Blood 100 (10): 3731-40, 2002. View Abstract

Chalandon Y, Jiang X, Hazlewood G, Loutet S, Conneally E, Eaves A, & Eaves C. Modulation of p210(BCR-ABL) activity in transduced primary human hematopoietic cells controls lineage programming. Blood 99 (9): 3197-204, 2002. View Abstract

Holyoake TL, Jiang X, Drummond MW, Eaves AC, & Eaves CJ. Elucidating critical mechanisms of deregulated stem cell turnover in the chronic phase of chronic myeloid leukemia. Leukemia 16 (4): 549-58, 2002. View Abstract

Cashman J, Clark-Lewis I, Eaves A, & Eaves C. Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice. Blood 99 (3): 792-9, 2002. View Abstract

Uchida N, Fujisaki T, Eaves AC, & Eaves CJ. Transplantable hematopoietic stem cells in human fetal liver have a CD34(+) side population (SP)phenotype. J Clin Invest 108 (7): 1071-7, 2001. View Abstract

Brummendorf TH, Rufer N, Holyoake TL, Maciejewski J, Barnett MJ, Eaves CJ, Eaves AC, Young N, & Lansdorp PM. Telomere length dynamics in normal individuals and in patients with hematopoietic stem cell-associated disorders. Ann N Y Acad Sci 938: 293-303; discussion 303-4, 2001. View Abstract

Holyoake TL, Jiang X, Jorgensen HG, Graham S, Alcorn MJ, Laird C, Eaves AC, & Eaves CJ. Primitive quiescent leukemic cells from patients with chronic myeloid leukemia spontaneously initiate factor-independent growth in vitro in association with up-regulation of expression of interleukin-3. Blood 97 (3): 720-8, 2001. View Abstract

Holyoake TL, Horrocks C, Thomas T, Eaves CJ, & Eaves AC. Cell separation improves the sensitivity of detecting rare human normal and leukemic hematopoietic cells in vivo in NOD/SCID mice. Cytotherapy 2 (6): 411-21, 2000. View Abstract

Jiang X, Fujisaki T, Nicolini F, Berger M, Holyoake T, Eisterer W, Eaves C, & Eaves A. Autonomous multi-lineage differentiation in vitro of primitive CD34+ cells from patients with chronic myeloid leukemia. Leukemia 14 (6): 1112-21, 2000. View Abstract

Hogge DE, Lambie K, Sutherland HJ, Benny WB, Dalal B, Currie C, Barnett MJ, Eaves AC, & Eaves CJ. Quantitation of primitive and lineage-committed progenitors in mobilized peripheral blood for prediction of platelet recovery post autologous transplant. Bone Marrow Transplant 25 (6): 589-98, 2000. View Abstract

Brummendorf TH, Holyoake TL, Rufer N, Barnett MJ, Schulzer M, Eaves CJ, Eaves AC, & Lansdorp PM. Prognostic implications of differences in telomere length between normal and malignant cells from patients with chronic myeloid leukemia measured by flow cytometry. Blood 95 (6): 1883-90, 2000. View Abstract

Cashman JD, Clark-Lewis I, Eaves AC, & Eaves CJ. Differentiation stage-specific regulation of primitive human hematopoietic progenitor cycling by exogenous and endogenous inhibitors in an in vivo model. Blood 94 (11): 3722-9, 1999. View Abstract

Jiang X, Lopez A, Holyoake T, Eaves A, & Eaves C. Autocrine production and action of IL-3 and granulocyte colony-stimulating factor in chronic myeloid leukemia. Proc Natl Acad Sci U S A 96 (22): 12804-9, 1999. View Abstract

Holyoake T, Jiang X, Eaves C, & Eaves A. Isolation of a highly quiescent subpopulation of primitive leukemic cells in chronic myeloid leukemia. Blood 94 (6): 2056-64, 1999. View Abstract

Ghaffari S, Smadja-Joffe F, Oostendorp R, Levesque JP, Dougherty G, Eaves A, & Eaves C. CD44 isoforms in normal and leukemic hematopoiesis. Exp Hematol 27 (6): 978-93, 1999. View Abstract

Eaves C, Miller C, Conneally E, Audet J, Oostendorp R, Cashman J, Zandstra P, Rose-John S, Piret J, & Eaves A. Introduction to stem cell biology in vitro. Threshold to the future. Ann N Y Acad Sci 872: 1-8, 1999. View Abstract

Eaves AC, Barnett MJ, Ponchio L, Cashman JD, Petzer AL, & Eaves CJ. Differences between normal and CML stem cells: potential targets for clinical exploitation. Stem Cells 16 Suppl 1: 77-83; discussion 89, 1998. View Abstract

Eaves C, Cashman J, & Eaves A. Defective regulation of leukemic hematopoiesis in chronic myeloid leukemia. Leuk Res 22 (12): 1085-96, 1998. View Abstract

Cashman JD, Eaves CJ, Sarris AH, & Eaves AC. MCP-1, not MIP-1alpha, is the endogenous chemokine that cooperates with TGF-beta to inhibit the cycling of primitive normal but not leukemic (CML) progenitors in long-term human marrow cultures. Blood 92 (7): 2338-44, 1998. View Abstract

Wang JC, Lapidot T, Cashman JD, Doedens M, Addy L, Sutherland DR, Nayar R, Laraya P, Minden M, Keating A, Eaves AC, Eaves CJ, & Dick JE. High level engraftment of NOD/SCID mice by primitive normal and leukemic hematopoietic cells from patients with chronic myeloid leukemia in chronic phase. Blood 91 (7): 2406-14, 1998. View Abstract

Maguer-Satta V, Burl S, Liu L, Damen J, Chahine H, Krystal G, Eaves A, & Eaves C. BCR-ABL accelerates C2-ceramide-induced apoptosis. Oncogene 16 (2): 237-48, 1998. View Abstract

Cashman J, Bockhold K, Hogge DE, Eaves AC, & Eaves CJ. Sustained proliferation, multi-lineage differentiation and maintenance of primitive human haemopoietic cells in NOD/SCID mice transplanted with human cord blood. Br J Haematol 98 (4): 1026-36, 1997. View Abstract

Carella AM, Frassoni F, Melo J, Sawyers C, Eaves C, Eaves A, Apperley J, Tura S, Hehlmann R, Reiffers J, Lerma E, & Goldman J. New insights in biology and current therapeutic options for patients with chronic myelogenous leukemia. Haematologica 82 (4): 478-95, 1997. View Abstract

Petzer AL, Eaves CJ, Barnett MJ, & Eaves AC. Selective expansion of primitive normal hematopoietic cells in cytokine-supplemented cultures of purified cells from patients with chronic myeloid leukemia. Blood 90 (1): 64-9, 1997. View Abstract

Eaves CJ, & Eaves AC. Stem cell kinetics. Baillieres Clin Haematol 10 (2): 233-57, 1997. View Abstract

Ghaffari S, Dougherty GJ, Eaves AC, & Eaves CJ. Diverse effects of anti-CD44 antibodies on the stromal cell-mediated support of normal but not leukaemic (CML) haemopoiesis in vitro. Br J Haematol 97 (1): 22-8, 1997. View Abstract

Hogge D, Fanning S, Bockhold K, Petzer A, Lambie K, Lansdorp P, Eaves A, & Eaves C. Quantitation and characterization of human megakaryocyte colony-forming cells using a standardized serum-free agarose assay. Br J Haematol 96 (4): 790-800, 1997. View Abstract

Eaves A. Stem cell heterogeneity (discussion). Stem Cells 15 Suppl 1: 217-20, 1997. View Abstract

Eaves C, Miller C, Cashman J, Conneally E, Petzer A, Zandstra P, & Eaves A. Hematopoietic stem cells: inferences from in vivo assays. Stem Cells 15 Suppl 1: 1-5, 1997. View Abstract

Ghaffari S, Dougherty GJ, Eaves AC, & Eaves CJ. Altered patterns of CD44 epitope expression in human chronic and acute myeloid leukemia. Leukemia 10 (11): 1773-81, 1996. View Abstract

Petzer AL, Eaves CJ, Lansdorp PM, Ponchio L, Barnett MJ, & Eaves AC. Characterization of primitive subpopulations of normal and leukemic cells present in the blood of patients with newly diagnosed as well as established chronic myeloid leukemia. Blood 88 (6): 2162-71, 1996. View Abstract

Maguer-Satta V, Petzer AL, Eaves AC, & Eaves CJ. BCR-ABL expression in different subpopulations of functionally characterized Ph+ CD34+ cells from patients with chronic myeloid leukemia. Blood 88 (5): 1796-804, 1996. View Abstract

Sirard C, Lapidot T, Vormoor J, Cashman JD, Doedens M, Murdoch B, Jamal N, Messner H, Addey L, Minden M, Laraya P, Keating A, Eaves A, Lansdorp PM, Eaves CJ, & Dick JE. Normal and leukemic SCID-repopulating cells (SRC) coexist in the bone marrow and peripheral blood from CML patients in chronic phase, whereas leukemic SRC are detected in blast crisis. Blood 87 (4): 1539-48, 1996. View Abstract

Eaves AC. Research versus regulation: striking a balance. J Hematother 5 (1): 1-2, 1996. View Abstract

Ghaffari S, Dougherty GJ, Lansdorp PM, Eaves AC, & Eaves CJ. Differentiation-associated changes in CD44 isoform expression during normal hematopoiesis and their alteration in chronic myeloid leukemia. Blood 86 (8): 2976-85, 1995. View Abstract

Sutherland HJ, Hogge DE, Lansdorp PM, Phillips GL, Eaves AC, & Eaves CJ. Quantitation, mobilization, and clinical use of long-term culture-initiating cells in blood cell autografts. J Hematother 4 (1): 3-10, 1995. View Abstract

Cashman JD, Eaves AC, & Eaves CJ. The tetrapeptide AcSDKP specifically blocks the cycling of primitive normal but not leukemic progenitors in long-term culture: evidence for an indirect mechanism. Blood 84 (5): 1534-42, 1994. View Abstract

Klingemann HG, Eaves CJ, Barnett MJ, Eaves AC, Hogge DE, Nantel SH, Reece E, Shepherd JD, Sutherland HJ, & Phillips GL. Transplantation of patients with high risk acute myeloid leukemia in first remission with autologous marrow cultured in interleukin-2 followed by interleukin-2 administration. Bone Marrow Transplant 14 (3): 389-96, 1994. View Abstract

Eaves C, & Eaves A. Differential manipulation of normal and chronic myeloid leukemia stem cell proliferation in vitro. Blood Cells 20 (1): 83-93; discussion 93-5, 1994. View Abstract

Cashman JD, Eaves AC, Wolpe S, & Eaves CJ. Regulatory activity of endogenous MIP-1 alpha in long-term cultures of human marrow. Prog Clin Biol Res 389: 261-8, 1994. View Abstract

Eaves CJ, Cashman JD, WolpeSD, & Eaves AC. Unresponsiveness of primitive chronic myeloid leukemia cells to macrophage inflammatory protein 1 alpha, an inhibitor of primitive normal hematopoietic cells. Proc Natl Acad Sci U S A 90 (24): 12015-9, 1993. View Abstract

Eaves CJ, Cashman JD, Zoumbos NC, Barnett MJ, & Eaves AC. Biological strategies for the selective manipulation of normal and leukemic stem cells. Stem Cells 11 Suppl 3: 109-21, 1993. View Abstract

Eaves CJ, Barnett MJ, & Eaves AC. In vitro culture of bone marrow cells for autografting in CML. Leukemia 7 Suppl 2: S126-9, 1993. View Abstract

Eaves AC, Eaves CJ, Phillips GL, & Barnett MJ. Culture purging in leukemia: past, present, and future. Leuk Lymphoma 11 Suppl 1: 259-63, 1993. View Abstract

Eaves C, Udomsakdi C, Cashman J, Barnett M, & Eaves A. The biology of normal and neoplastic stem cells in CML. Leuk Lymphoma 11 Suppl 1: 245-53, 1993. View Abstract

Udomsakdi C, Eaves CJ, Lansdorp PM, & Eaves AC. Phenotypic heterogeneity of primitive leukemic hematopoietic cells in patients with chronic myeloid leukemia. Blood 80 (10): 2522-30, 1992. View Abstract

Udomsakdi C, Lansdorp PM, Hogge DE, Reid DS, Eaves AC, & Eaves CJ. Characterization of primitive hematopoietic cells in normal human peripheral blood. Blood 80 (10): 2513-21, 1992. View Abstract

Cashman JD, Eaves AC, & Eaves CJ. Granulocyte-macrophage colony-stimulating factor modulation of the inhibitory effect of transforming growth factor-beta on normal and leukemic human hematopoietic progenitor cells. Leukemia 6 (9): 886-92, 1992. View Abstract

Turhan AG, Cashman JD, Eaves CJ, Humphries RK, & Eaves AC. Variable expression of features of normal and neoplastic stem cells in patients with thrombocytosis. Br J Haematol 82 (1): 50-7, 1992. View Abstract

Udomsakdi C, Eaves CJ, Swolin B, Reid DS, Barnett MJ, & Eaves AC. Rapid decline of chronic myeloid leukemic cells in long-term culture due to a defect at the leukemic stem cell level. Proc Natl Acad Sci U S A 89 (13): 6192-6, 1992. View Abstract

Emanuel PD, Eaves CJ, Broudy VC, Papayannopoulou T, Moore MR, D’Andrea AD, Prchal JF, Eaves AC, & Prchal JT. Familial and congenital polycythemia in three unrelated families. Blood 79 (11): 3019-30, 1992. View Abstract

Shepherd JD, Currie CJ, Sparling TG, Krystal G, & Eaves AC. Erythropoietin therapy of myelodysplastic syndromes. Blood 79 (7): 1891-2, 1992. View Abstract

Wognum AW, Krystal G, Eaves CJ, Eaves AC, & Lansdorp PM. Increased erythropoietin-receptor expression on CD34-positive bone marrow cells from patients with chronic myeloid leukemia. Blood 79 (3): 642-9, 1992. View Abstract

Eaves C, Fraser C, Udomsakdi C, Sutherland H, Barnett M, Szilvassy S, Hogge D, Lansdorp P, & Eaves A. Manipulation of the hematopoietic stem cell in vitro. Leukemia 6 Suppl 1: 27-30, 1992. View Abstract

Eaves CJ, Sutherland HJ, Udomsakdi C, Lansdorp PM, Szilvassy SJ, Fraser CC, Humphries RK, Barnett MJ, Phillips GL, & Eaves AC. The human hematopoietic stem cell in vitro and in vivo. Blood Cells 18 (2): 301-7, 1992. View Abstract

Klingemann HG, Grigg AP, Wilkie-Boyd K, Barnett MJ, Eaves AC, Reece DE, Shepherd JD, & Phillips GL. Treatment with recombinant interferon (alpha-2b) early after bone marrow transplantation in patients at high risk for relapse [corrected]. Blood 78 (12): 3306-11, 1991. View Abstract

Otsuka T, Eaves CJ, Humphries RK, Hogge DE, & Eaves AC. Lack of evidence for abnormal autocrine or paracrine mechanisms underlying the uncontrolled proliferation of primitive chronic myeloid leukemia progenitor cells. Leukemia 5 (10): 861-8, 1991. View Abstract

Otsuka T, Humphries RK, Hogge DE, Eaves AC, & Eaves CJ. Continuous activation of primitive hematopoietic cells in long-term human marrow cultures containing irradiated tumor cells. J Cell Physiol 148 (3): 370-9, 1991. View Abstract

Turhan AG, Eaves CJ, Humphries RK, Phillips GL, & Eaves AC. Reversing clonality in leukemia. Semin Hematol 28 (3 Suppl 4): 5-8, 1991. View Abstract

Eaves CJ, Cashman JD, Kay RJ, Dougherty GJ, Otsuka T, Gaboury LA, Hogge DE, Lansdorp PM, Eaves AC, & Humphries RK. Mechanisms that regulate the cell cycle status of very primitive hematopoietic cells in long-term human marrow cultures. II. Analysis of positive and negative regulators produced by stromal cells within the adherent layer. Blood 78 (1): 110-7, 1991. View Abstract

Eaves CJ, Sutherland HJ, Cashman JD, Otsuka T, Lansdorp PM, Humphries RK, Eaves AC, & Hogge DE. Regulation of primitive human hematopoietic cells in long-term marrow culture. Semin Hematol 28 (2): 126-31, 1991. View Abstract

Barnett MJ, Sutherland HJ, Eaves AC, Hogge DE, Humphries RK, Klingemann HG, Lansdorp PM, Phillips GL, Reece DE, Shepherd JD, & et al. Human hematopoietic stem cells in long-term culture: quantitation and manipulation. Bone Marrow Transplant 7 Suppl 1: 70, 1991. View Abstract

Klingemann HG, Shepherd JD, Eaves CJ, & Eaves AC. The role of erythropoietin and other growth factors in transfusion medicine. Transfus Med Rev 5 (1): 33-47, 1991. View Abstract

Eaves CJ, Cashman JD, Sutherland HJ, Otsuka T, Humphries RK, HoggeDE, Lansdorp PL, & Eaves AC. Molecular analysis of primitive hematopoietic cell proliferation control mechanisms. Ann N Y Acad Sci 628: 298-306, 1991. View Abstract

Szilvassy SJ, Humphries RK, Lansdorp PM, Eaves AC, & Eaves CJ. Quantitative assay for totipotent reconstituting hematopoietic stem cells by a competitive repopulation strategy. Proc Natl Acad Sci U S A 87 (22): 8736-40, 1990. View Abstract

Barnett MJ, Eaves AC, & Phillips GL. An overview of bone marrow transplantation for chronic myeloid leukemia. Cmaj 143 (3): 187-93, 1990. View Abstract

Sutherland HJ, Lansdorp PM, Henkelman DH, Eaves AC, & Eaves CJ. Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow stromal layers. Proc Natl Acad Sci U S A 87 (9): 3584-8, 1990. View Abstract

Klingemann HG, Eaves AC, Barnett MJ, Reece DE, Shepherd JD, Belch AR, Brandwein JM, Langleben A, Koch PA, & Phillips GL. Recombinant GM-CSF in patients with poor graft function after bone marrow transplantation. Clin Invest Med 13 (2): 77-81, 1990. View Abstract

Cashman JD, Eaves AC, Raines EW, Ross R, & Eaves CJ. Mechanisms that regulate the cell cycle status of very primitive hematopoietic cells in long-term human marrow cultures. I. Stimulatory role of a variety of mesenchymal cell activators and inhibitory role of TGF-beta. Blood 75 (1): 96-101, 1990. View Abstract

Brandwein JM, HorsmanDE, Eaves AC, Eaves CJ, Massing BG, Wadsworth LD, Rogers PC, & Kalousek DK. Childhood myelodysplasia: suggested classification as myelodysplastic syndromes based on laboratory and clinical findings. Am J Pediatr Hematol Oncol 12 (1): 63-70, 1990. View Abstract

Eaves AC, & Eaves CJ. Growth control in leukemia. Prog Clin Biol Res 354A: 223-36, 1990. View Abstract

Szilvassy SJ, Fraser CC, Eaves CJ, Lansdorp PM, Eaves AC, & Humphries RK. Retrovirus-mediated gene transfer to purified hemopoietic stem cells with long-term lympho-myelopoietic repopulating ability. Proc Natl Acad Sci U S A 86 (22): 8798-802, 1989. View Abstract

Sutherland HJ, Eaves CJ, Eaves AC, Dragowska W, & Lansdorp PM. Characterization and partial purification of human marrow cells capable of initiating long-term hematopoiesis in vitro. Blood 74 (5): 1563-70, 1989. View Abstract

Shannon KM, Turhan AG, Chang SS, Bowcock AM, Rogers PC, Carroll WL, Cowan MJ, Glader BE, Eaves CJ, Eaves AC, & et al. Familial bone marrow monosomy 7. Evidence that the predisposing locus is not on the long arm of chromosome 7. J Clin Invest 84 (3): 984-9, 1989. View Abstract

Szilvassy SJ, Lansdorp PM, Humphries RK, Eaves AC, & Eaves CJ. Isolation in a single step of a highly enriched murine hematopoietic stem cell population with competitive long-term repopulating ability. Blood 74 (3): 930-9, 1989. View Abstract

Wognum AW, Lansdorp PM, Eaves AC, & Krystal G. An enzyme-linked immunosorbent assay for erythropoietin using monoclonal antibodies, tetrameric immune complexes, and substrate amplification. Blood 74 (2): 622-8, 1989. View Abstract

Barnett MJ, Eaves CJ, Phillips GL, Kalousek DK, Klingemann HG, Lansdorp PM, Reece DE, Shepherd JD, Shaw GJ, & Eaves AC. Successful autografting in chronic myeloid leukaemia after maintenance of marrow in culture. Bone Marrow Transplant 4 (4): 345-51, 1989. View Abstract

Turhan AG, Humphries RK, Phillips GL, Eaves AC, & Eaves CJ. Clonal hematopoiesis demonstrated by X-linked DNA polymorphisms after allogeneic bone marrow transplantation. N Engl J Med 320 (25): 1655-61, 1989. View Abstract

Turhan AG, Eaves CJ, Connors JM, Eaves AC, Karim KA, & Silver KB. Hematologic and immunologic changes in hairy cell leukemia patients treated with alpha interferon. J Biol Regul Homeost Agents 2 (4): 157-65, 1988. View Abstract

Kalousek DK, Dube ID, Eaves CJ, & Eaves AC. Cytogenetic studies of haemopoietic colonies from patients with an initial diagnosis of acute lymphoblastic leukaemia. Br J Haematol 70 (1): 5-11, 1988. View Abstract

Eaves CJ, & Eaves AC. Regulation of hemopoietic progenitor cell proliferation. Behring Inst Mitt  (83): 85-92, 1988. View Abstract

Turhan AG, Humphries RK, Cashman JD, Cuthbert DA, Eaves CJ, & Eaves AC. Transient suppression of clonal hemopoiesis associated with pregnancy in a patient with a myeloproliferative disorder. J Clin Invest 81 (6): 1999-2003, 1988. View Abstract

Turhan AG, Eaves CJ, Kalousek DK, Eaves AC, & Humphries RK. Molecular analysis of clonality and bcr rearrangements in Philadelphia chromosome-positive acute lymphoblastic leukemia. Blood 71 (5): 1495-8, 1988. View Abstract

Cashman JD, Eaves CJ, & Eaves AC. Unregulated proliferation of primitive neoplastic progenitor cells in long-term polycythemia vera marrow cultures. J Clin Invest 81 (1): 87-91, 1988. View Abstract

Eaves AC, & Eaves CJ. Maintenance and proliferation control of primitive hemopoietic progenitors in long-term cultures of human marrow cells. Blood Cells 14 (2-3): 355-68, 1988. View Abstract

Eaves CJ, & Eaves AC. Cell culture studies in CML. Baillieres Clin Haematol 1 (4): 931-61, 1987. View Abstract

Hogge DE, Coulombel L, Kalousek DK, Eaves CJ, & Eaves AC. Nonclonal hemopoietic progenitors in a G6PD heterozygote with chronic myelogenous leukemia revealed after long-term marrow culture. Am J Hematol 24 (4): 389-94, 1987. View Abstract

Hogge DE, Shannon KM, Kalousek DK, Schonberg S, Schaffner V, Zoger S, Eaves CJ, & Eaves AC. Juvenile monosomy 7 syndrome: evidence that the disease originates in a pluripotent hemopoietic stem cell. Leuk Res 11 (8): 705-9, 1987. View Abstract

Eaves AC, Cashman JD, Gaboury LA, & Eaves CJ. Clinical significance of long-term cultures of myeloid blood cells. Crit Rev Oncol Hematol 7 (2): 125-38, 1987. View Abstract

Howard DR, Eaves AC, & Takei F. Monoclonal antibodies to HLA-DP, DQ, and DR determinants: functional effects on the activation and proliferation of normal and EBV-transformed B cells. Exp Hematol 14 (9): 887-95, 1986. View Abstract

Eaves AC, Cashman JD, Gaboury LA, Kalousek DK, & Eaves CJ. Unregulated proliferation of primitive chronic myeloid leukemia progenitors in the presence of normal marrow adherent cells. Proc Natl Acad Sci U S A 83 (14): 5306-10, 1986. View Abstract

Howard DR, Eaves AC, & Takei F. Lymphocyte function-associated antigen (LFA-1) is involved in B cell activation. J Immunol 136 (11): 4013-8, 1986. View Abstract

Askew DS, Eaves AC, Eaves CJ, & Takei F. Restricted expression of a new acute myelogenous leukemia-associated antigen (NHL-30.5) on normal hemopoietic progenitor cells. Blood 67 (4): 1098-102, 1986. View Abstract

Howard DR, Eaves AC, & Takei F. Two monoclonal antibodies defining unique antigenic determinants on human B-lymphoma cells. Cancer Res 45 (12 Pt 1): 6322-7, 1985. View Abstract

Kerk DK, Henry EA, Eaves AC, & Eaves CJ. Two classes of primitive pluripotent hemopoietic progenitor cells: separation by adherence. J Cell Physiol 125 (1): 127-34, 1985. View Abstract

Cashman J, Eaves AC, & Eaves CJ. Regulated proliferation of primitive hematopoietic progenitor cells in long-term human marrow cultures. Blood 66 (4): 1002-5, 1985. View Abstract

Coulombel L, Eaves C, Kalousek D, Gupta C, & Eaves A. Long-term marrow culture of cells from patients with acute myelogenous leukemia. Selection in favor of normal phenotypes in some but not all cases. J Clin Invest 75 (3): 961-9, 1985. View Abstract

Eaves C, Coulombel L, Dube I, Kalousek D, Cashman J, & Eaves A. Maintenance of normal and abnormal hemopoietic cell populations in long-term cultures of CML and AML marrow cells. Prog Clin Biol Res 184: 403-13, 1985. View Abstract

Eaves C, Coulombel L, Dube I, Kalousek D, Cashman J, & Eaves A. Behavior of human leukemic progenitor populations in long-term marrow culture. Haematol Blood Transfus 29: 163-7, 1985. View Abstract

Askew DS, Eaves AC, & Takei F. NHL-30.5: a monoclonal antibody reactive with an acute myeloid leukemia (AML)-associated antigen. Leuk Res 9 (1): 135-45, 1985. View Abstract

Dube ID, Arlin ZA, Kalousek DK, Eaves CJ, & Eaves AC. Nonclonal hemopoietic progenitor cells detected in long-term marrow cultures from a Turner syndrome mosaic with chronic myeloid leukemia. Blood 64 (6): 1284-7, 1984. View Abstract

Krystal G, Eaves CJ, & Eaves AC. CM Affi-Gel Blue chromatography of human urine: a simple one-step procedure for obtaining erythropoietin suitable for in vitro erythropoietic progenitor assays. Br J Haematol 58 (3): 533-46, 1984. View Abstract

Eaves AC, & Eaves CJ. Erythropoiesis in culture. Clin Haematol 13 (2): 371-91, 1984. View Abstract

Dube ID, Kalousek DK, Coulombel L, Gupta CM, Eaves CJ, & Eaves AC. Cytogenetic studies of early myeloid progenitor compartments in Ph1-positive chronic myeloid leukemia. II. Long-term culture reveals the persistence of Ph1-negative progenitors in treated as well as newly diagnosed patients. Blood 63 (5): 1172-7, 1984. View Abstract

Dube ID, Gupta CM, Kalousek DK, Eaves CJ, & Eaves AC. Cytogenetic studies of early myeloid progenitor compartments in Ph1-positive chronic myeloid leukaemia (CML). I. Persistence of Ph1-negative committed progenitors that are suppressed from differentiating in vivo. Br J Haematol 56 (4): 633-44, 1984. View Abstract

Coulombel L, Eaves CJ, Dube ID, Kalousek DK, & Eaves AC. Variable persistence of leukemic progenitor cells in long-term CML and AML marrow cultures. Kroc Found Ser 18: 243-54, 1984. View Abstract

Eaves CJ, Krystal G, & Eaves AC. Erythropoietic cells. Bibl Haematol  (48): 81-111, 1984. View Abstract

Eaves CJ, & Eaves AC. Factors and hemopoiesis: Pandora’s box revisited. Prog Clin Biol Res 148: 83-92, 1984. View Abstract

Coulombel L, Eaves AC, & Eaves CJ. Enzymatic treatment of long-term human marrow cultures reveals the preferential location of primitive hemopoietic progenitors in the adherent layer. Blood 62 (2): 291-7, 1983. View Abstract

Coulombel L, Kalousek DK, Eaves CJ, Gupta CM, & Eaves AC. Long-term marrow culture reveals chromosomally normal hematopoietic progenitor cells in patients with Philadelphia chromosome-positive chronic myelogenous leukemia. N Engl J Med 308 (25): 1493-8, 1983. View Abstract

Cashman J, Henkelman D, Humphries K, Eaves C, & Eaves A. Individual BFU-E in polycythemia vera produce both erythropoietin dependent and independent progeny. Blood 61 (5): 876-84, 1983. View Abstract

Dube ID, Eaves CJ, Kalousek DK, & Eaves AC. A method for obtaining high quality chromosome preparations from single hemopoietic colonies on a routine basis. Cancer Genet Cytogenet 4 (2): 157-68, 1981. View Abstract

Humphries RK, Eaves AC, & Eaves CJ. Self-renewal of hemopoietic stem cells during mixed colony formation in vitro. Proc Natl Acad Sci U S A 78 (6): 3629-33, 1981. View Abstract

Krystal G, Eaves AC, & Eaves CJ. Determination of normal human serum erythropoietin levels, using mouse bone marrow. J Lab Clin Med 97 (2): 158-69, 1981. View Abstract

Krystal G, Eaves AC, & Eaves CJ. A quantitative bioassay for erythropoietin, using mouse bone marrow. J Lab Clin Med 97 (2): 144-57, 1981. View Abstract

Eaves AC, Henkelman DH, & Eaves CJ. Abnormal erythropoiesis in the myeloproliferative disorders: an analysis of underlying cellular and humoral mechanisms. Exp Hematol 8 Suppl 8: 235-47, 1980. View Abstract

Eaves CJ, Humphries RK, & Eaves AC. Marrow flask cultures—a system for examining early erythropoietic differentiation events. Blood Cells 5 (3): 377-87, 1979. View Abstract

Humphries RK, Jacky PB, Dill FJ, Eaves AC, & Eaves CJ. CFU-S in individual erythroid colonies derived in vitro from adult mouse marrow. Nature 279 (5715): 718-20, 1979. View Abstract

Humphries RK, Eaves AC, & Eaves CJ. Characterization of a primitive erythropoietic progenitor found in mouse marrow before and after several weeks in culture. Blood 53 (4): 746-63, 1979. View Abstract

Eaves AC, & Eaves CJ. Abnormalities in the erythroid progenitor compartments in patients with chronic myelogenous leukemia (CML). Exp Hematol 7 Suppl 5: 65-75, 1979. View Abstract

Eaves CJ, & Eaves AC. Erythroid progenitor cell numbers in human marrow - implications for regulation. Exp Hematol 7 Suppl 5: 54-64, 1979. View Abstract

Eaves CJ, & Eaves AC. Erythropoietin (Ep) dose-response curves for three classes of erythroid progenitors in normal human marrow and in patients with polycythemia vera. Blood 52 (6): 1196-210, 1978. View Abstract

Gregory CJ, & Eaves AC. Three stages of erythropoietic progenitor cell differentiation distinguished by a number of physical and biologic properties. Blood 51 (3): 527-37, 1978. View Abstract

Gregory CJ, & Eaves AC. Human marrow cells capable of erythropoietic differentiation in vitro: definition of three erythroid colony responses. Blood 49 (6): 855-64, 1977. View Abstract

Eaves AC, & Bruce WR. [Altered sensitivity of hematopoietic stem cells to 5-fluorouracil (NSC-19893) following endotoxin (NSC-189681), cyclophosphamide (NSC-26271), or irradiation.]. Cancer Chemother Rep 58 (6): 813-20, 1974. View Abstract

Eaves AC, & Bruce WR. In vitro production of colony-stimulating activity. I. Exposure of mouse peritoneal cells to endotoxin. Cell Tissue Kinet 7 (1): 19-30, 1974. View Abstract

Eaves AC, & Bruce WR. Endotoxin-induced sensitivity of hematopoietic stem cells to chemotherapeutic agents. Ser Haematol 5 (2): 64-72, 1972. View Abstract

Eaves A, & Macaulay RC. Sulphur Dioxide Pollution: Statistical Analysis of Results from Adjacent Lead Dioxide and Hydrogen Peroxide Instruments. Air Water Pollut 8: 645-55, 1964. View Abstract

Newall HE, & Eaves A. The effect of wind speed and rainfall on the concentration of sulphur dioxide in the atmosphere. Int J Air Water Pollut 6: 173-7, 1962. View Abstract