Principal Investigators


Dr. Pamela Hoodless — PhD

Director & Distinguished Scientist

Laboratory Contact

Rebecca Cullum

Cancer Research Technologist
  604.675.8000 Ext. 7724

Admin Contact

Jordan Cran

Research Projects Manager
Research Interest
Lab Members
  • Professor, Medical Genetics, University of British Columbia
  • Professor, School of Biomedical Engineering, University of British Columbia
  • Faculty Member, Genome Science & Technology Graduate Program, University of British Columbia
  • Faculty Member, Developmental and Cell Biology, University of British Columbia
  • Associate Member, Bioinformatics Training Program, CIHR
  • Associate Member, Department of Pathology, University of British Columbia

My laboratory uses state of the art genomic technologies to shed new light on classical processes in developmental biology. We are exploring regulatory mechanisms in heart and gastrointestinal tract development in the mouse embryo using genome-wide analysis of gene expression, transcription factor binding, and histone modifications. We have two main areas of focus:

Organogenesis in the definitive endoderm

The control of gene expression during liver differentiation

The liver is a fascinating organ which controls a wide-array of homeostatic processes in the body including detoxification, metabolism and bile production. Liver formation initiates as a bud that evaginates from the foregut endoderm in response to signals from the neighbouring cardiac mesoderm and the septum transversum. This bud grows and differentiates to form the functional liver.

We are interested in the process of gene regulation during liver formation from the endoderm through to the adult organ.  We are using sequenced-based gene expression methods to analyze changes in gene expression patterns throughout differentiation of hepatocytes, the primary functional cell of the liver. We are correlating gene expression changes with transcriptional regulation by using chromatin immunoprecipiation coupled with next generation sequencing (ChIP-seq) to examine the patterns of transcription factor binding and histone modifications in the embryonic hepatoblast and adult hepatocyte. Analysis of microRNAs in this same process is also ongoing. Our work will investigate the transcriptional networks and mechanisms that function during hepatocyte differentiation.

Signaling pathways that control midgut formation

The midgut is the region of the definitive endoderm that will form part of the pancreas and the intestine. By using gene expression analysis, we have identified a series of novel genes that have distinct patterns of expression in the endoderm. One of these genes, Nephrocan, is exclusively expressed in the midgut of the early embryo. We are using this gene to explore the signaling mechanisms controlling midgut formation in the mouse embryo.

Our work will provide insights into the origins and functions of normal and cancer stem cells of the intestine and liver. Moreover, there is growing interest in differentiating pluripotent stem cells from many different sources in the laboratory to develop tissues for drug testing and transplantation. Understanding the normal in vivo differentiation processes will contribute to the development of better methodologies for the differentiation of cells in the laboratory for these purposes.

Heart valve formation

Congenital heart malformations are the most common form of birth defects, with many of these involving the heart valves. In the embryonic heart, the valves develop in the region between the atria and the ventricles, known as the atrioventricular canal (AVC) and in the region where blood flows into the major arteries, known as the outflow tract (OFT). Valve formation initiates in these regions when cells of the endocardium lining of the heart, undergo endothelial-to-mesenchymal transition (EMT) and migrate into the region, known as the endocardial cushion, located between the endocardium and the myocardium. The cushions then undergo remodeling through cellular differentiation to form the valve leaflets and these cells secrete the extracellular matrix that provides the mechanical flexibility to the valve leaflets.

Our work is focusing on the transcriptional networks that control valve leaflet formation during the early stages of valve formation. As with the liver, we are using cutting edge gene expression analysis methodology and ChIP-seq to examine functions of transcription factors and histone modifications in the control of valve formation.

Of interest, valve calcification in adults has been linked to the same pathways that control valve formation in the embryo. Our work will provide insights into potential targets of congenital heart defects as well as adult heart valve disease. In addition, our work will contribute to a greater understanding of EMT, which is the major driver of cancer metastases.

Selected Publications

Coulombe P, Paliouras GN, Clayton A, Hussainkhel A, Fuller M, Jovanovic V, Dauphinee S, Umlandt P, Xiang P, Kyle AH, Minchinton AI, Humphries RK, Hoodless PA, Parker JDK, Wright JL, Karsan A. Endothelial Sash1 Is Required for Lung Maturation through Nitric Oxide Signaling. Cell Reports. 27:1769-1780, 2019. View Abstract

Nowotschin S, Setty M, Kuo YY, Liu V, Garg V, Sharma R, Simon CS, Saiz N, Gardner R, Boutet SC, Church DM, Hoodless PA, Hadjantonakis AK, Pe'er D. The emergent landscape of the mouse gut endoderm at single-cell resolution. Nature. 2019 Apr 8.  View Abstract [Epub ahead of print]

Thakur A, Wong JCH, Wang EY, Lotto J, Kim D, Cheng JC, Mingay M, Cullum R, Moudgil V, Ahmed N, Tsai SH, Wei W, Walsh CP, Stephan T, Bilenky M, Fuglerud BM, Karimi MM, Gonzalez FJ, Hirst M, Hoodless PA. HNF4A is essential for the active epigenetic state at enhancers in mouse liver. Hepatology. 2019 Apr 1. View Abstract [Epub ahead of print]

Wang EY, Cheng J-C, Thakur A,Yi Y, Tsai S-H, Hoodless PA. YAP transcriptionally regulates ErbB2 to promote liver cell proliferation. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms. 1861(9): 854-863, 2018. View Abstract

Cheng J-C, Wang EY, Yi Y, Thakur A, Tsai S-H, Hoodless PA. S1P Stimulates Proliferation by Up-regulating CTGF Expression through S1PR2-mediated YAP Activation. Molecular Cancer Research. 16(10): 1543-1555, 2018. View Abstract

Wei W, Lotto J, Hoodless PA. Expression patterns of Yes-associated protein 1 in the developing mouse liver. Gene Expr Patterns. 29: 10-17, 2018. View Abstract

Xiang P, Wei W, Hoff N, Clemans-Gibbon J, Maetzig T, Lai C, Dhillon I, May C, Rushmann J, Rosten P, Hu K, Hoodless PA, Humphries RK. A knock-in Meis1 mouse strain facilitates dynamic tracking and enrichment of Meis1. Blood Adv. 24(1): 2225-2235, 2017. View Abstract

Yi Y, Tsai S-H, Cheng J-C, Wang EY, Anglesio MS, Cochrane DR, Fuller M, Gibb EA, Wei W, Huntsman DG, Karsan A, Hoodless PA. APELA promotes tumour growth and cell migration in ovarian cancer in a p53-dependent manner. Gynecologic Oncology. 147(3): 663-671, 2017. View Abstract

Freyer L, Hsu C-W, Nowotschin S, Pauli A, Ishida J, Kuba K, Fukamizu A, Schier AF, Hoodless PA, Dickinson ME, Hadjantonakis A-K. Loss of Apela peptide in mice causes low penetrance embryonic lethality and defects in early mesodermal derivatives. Cell Reports. 20(9):2116-2130, 2017. View Abstract

Wilcox SM, Arora H, Munro L, Xin J, Fenninger F, Johnson LA, Pfiefer CG, Choi KB, Hou J, Hoodless PA, Jefferies WA. The Role of the Innate Immune Response Regulatory Gene ABCF1 in Mammalian Embryogenesis and Development.  PlosOne.  12(5):e0175918, 2017. View Abstract

Garside VC, Cullum R, Alder O, Lu DY, Vander Werff R, Bilenky M, Zhao Y, Jones SJM, Marra MA, Underhill TM, Hoodless PA. SOX9 modulates the expression of key transcription factors required for heart valve development. Development. 142: 4340-4350, 2015. View Abstract

Pon J, Wong J, Saberi S, Alder O, Moksa M, Cheng SWG, Morin G, Hoodless PA, Hirst M & Marra, M. MEF2B mutations in non-Hodgkin lymphoma dysregulate cell migration by decreasing MEF2B target gene activation. Nat Commun. 6:7953. 2015 View Abstract

Sanders SS, Hou J, Sutton LM, Garside, VC, Mui KK, Singaraja RR, Hayden MR & Hoodless PA. Huntingtin interacting protein 14 and 14-like are required for chorioallantoic fusion during early placental development. Dev Biol. 397(2):257-66, 2015. View Abstract

Alder O, Cullum R, Lee S, Kan AC, Wei W, Yi Y, Garside VC, Bilenky M, Griffiths M, Morrissy AS, , Robertson GA, Thiessen N, Zhao YJ, Chen Q, Pan D, Jones SJM, Marra MA, Hoodless PA. Hippo signalling influences HNF4A and FOXA2 enhancer switching during hepatocyte differentiation. Cell Rep. 9(1):261-71, 2014.  View Abstract

Hou J, Wei W, Saund RS, Xiang P, Cunningham TJ, Yi Y, Lu DYD, Savory JGA, Krentz NAJ, Montpetit R, Cullum R, Lohnes D, Humphries RK, Yamanaka Y, Duester G, Saijoh Y, Hoodless PA. A regulatory network controls Nephrocan expression and midgut patterning.  Development. 141(19):3772-81, 2014. View Abstract

Chang ACY, Garside VC, Fournier M, Smrz J, Vrljicak P, Umlandt P, Fuller M, Robertson G, Zhao Y, Tam A, Jones SJ, Marra MA, Hoodless PA, Karsan A. A Notch-dependent transcriptional hierarchy promotes mesenchymal transdifferentiation in the cardiac cushion. Dev Dyn. 243:894-905, 2014 View Abstract

Ye Y, Zhang HF, Qiu Y, Hanson PJ, Hemida MJ, Wei W, Hoodless P, Chu F & Yang D. Coxsackievirus-induced miR-21 disrupts cardiomyocyte interactions via the downregulation of intercalated disk components. PLoS Pathog 10: e1004070, 2014. View Abstract

Xiang P, Wei W, Lo C, Rosten P, Hou J, Hoodless PA, Bilenky M, Bonifer C, Cockeril PN, Kirkpatrick A, Gottgens B, Hirst M, Humphries KR. Delineating MEIS1 cis-regulatory elements active in hematopoietic cells. Leukemia, 28:433–436, 2014. View Abstract

Ye X, Zhang HM, Qiu Y, Hanson PJ, Hemida MG, Wei W, Hoodless PA, Chu F, Yang D. Coxsackievirus-induced miR-21 disrupts cardiomyocyte interactions via the downregulation of intercalated disk components. PLoS Pathog. 10:e1004070, 2014 View Abstract

Swanson L, Robertson G, Mungall KL, Butterfield YS, Chiu R, Corbett RD, Docking TR, Hogge D, Jackman SD, Moore RA, Mungall AJ, Nip KM, Parker JD, Qian JQ, Raymond A, Sung S, Tam A, Thiessen N, Varhol R, Wang S, Yorukoglu D, Zhao Y, Hoodless PA, Sahinalp SC, Karsan A, Birol I. Barnacle: detecting and characterizing tandem duplications and fusions in transcriptome assemblies. BMC Genomics 14:550, 2013 View Abstract

Wei W, Hou J, Alder O, Ye X, Lee S, Cullum R, Chu A, Zhao Y, Warner SM, Knight DA, Yang D, Jones SJ, Marra MA, Hoodless PA. Genome-wide microRNA and mRNA profiling in mouse liver development implicates mir302b and mir20a in repressing TGFβ signaling. Hepatology 57:2491-501,2013. View Abstract

Tennant BR, Robertson G, Kramer M, Li L, Zhang X, Beach M, Thiessen N, Chiu R, Mungall K, Whiting CJ, Sabatini P, Kim A, Gottardo R, Marra MA, Lynn F, Jones SJM, Hoodless PA, Hoffman BG. Identification and analysis of murine pancreatic islet enhancers. Diabetologica 56: 542-52, 2013. View Abstract

Garside VC, Chang ACY, Karsan A, Hoodless PA. Co-ordinating Notch, BMP, and TGFβ signalling during heart valve development. Cell Mol Life Sci [epub ahead of print Nov 16], 2012. Invited Review View Abstract

Vrljicak P, CullumR, Xu E, Chang ACY, Wederell ED, Bilenky M, Jones SJM, Marra MA, Karsan A, Hoodless PA. Twist1 transcriptional targets in the developing atrio-ventricular canal of the mouse. PLoS One 7:e40815, 2012. View Abstract

Hneio M, Blirando K, Buard V, Tarlet G, Benderitter, Hoodless PA, Francois A, Milliat F. The TG-interacting factor TGIF1 regulates stress-induced pro-inflammatory phenotype of endothelial cells. J Biol Chem 287: 38913-21, 2012. View Abstract

Hitz MP, Lemieux-Perrault LP, Marshall CR, ZadaYF, Davies R, Yang SW, Lionel AC, D’Amours G, Lemyre E, Cullum R, Bigras JL, Thibeault M, Chetaille P, Montpetit A, Khairy P, Overduin B, Klaassen S, Hoodless P, Nemer M, Stewart AFR, Boerkoel C, Scherer SW, Richter A, Dube MP, Andelfinger G. Rare copy number variants contribute to congenital left-sided heart disease. PLoS Genet 8: e1002903, 2012. View Abstract

Hneino M, Francois A, Buard V, Tarlet G, Abderrahmani R, Blirando K, Hoodless PA, Benderitter M, Milliat F. The TGF-b/Smad repressor TG-interacting factor 1 (TGIF1) plays a role in radiation-induced-intestinal injury independently of a Smad signaling pathway. PLoS One 7: e35672, 2012. View Abstract

Yusuf D, Butland SL, Swanson MI, Bolotin E, Ticoll A, Cheung WA, Yu XY, Zhang C, Dickman CTD, Fulton DL, Lim JS, Schnabl JM, Ramos OHP, Vasseur-Cognet M, de Leeuw CN, Simpson EM, Ryffel GU, Lam EWF, Kist R, Wilson MSC, Marco-Ferreres R, Brosens JJ, Beccari LL, Bovolenta P, Benayoun BA, Monteiro LJ, Schwenen HDC, Grontved L, Wederell E, Mandrup S, Veitia RA, Chakravarthy H, Hoodless PA, Mancarelli MM, Torbett BE, Banham AH, Reddy SP, Cullum RL, Liedtke M, Tschan MP, Vaz M, Rizzino A, Zannini M, Frietze S, Farnham PJ, Eijkelenboom A, Brown PJ, Laperrière D, Leprince D, de Cristofaro T, Prince KL, Putker M, del Peso L, Camenisch G, Wenger RH, Mikula M, Rozendaal M, Mader S, Ostrowski J, Rhodes SJ, Van Rechem C, Boulay G, Olechnowicz SWZ, Breslin MB, Lan MS, Nanan KK, Wegner M, Hou J, Mullen RD, Colvin SC, Noy PJ, Webb CF, Witek ME, Ferrell S, Daniel JM, Park J, Waldman SA,  Peet DJ, Taggart M, Jayaraman P-S, Karrich JJ, Blom B, Vesuna F, O'Geen H, Sun Y, Gronostajski RM, Woodcroft MW, Hough MR, Chen E, Europe-Finner GN, Karolczak-Bayatti M, Bailey J, Hankinson O, Raman V, LeBrun DP, Biswal S, Harvey CJ, DeBruyne JP, Hogenesch JB, Hevner RF, Héligon C, Luo XM, Blank MC, Millen KJ, Sharlin DS, Forrest D, Dahlman-Wright K, Zhao C, Mishima Y, Sinha S, Chakrabarti R, Portales-Casamar E, Sladek FM, Bradley PH and Wasserman WW. The Transcription Factor Encyclopedia. Genome Biol 13:R24, 2012. View Abstract

Chang AC, Fu Y, Garside VC, Niessen K, Chang L, Fuller M, Setiadi A, Smrz J, Kyle A, Minchinton A, Marra M, Hoodless PA, Karsan A. Notch Initiates the Endothelial-to-Mesenchymal Transition in the Atrioventricular Canal through Autocrine Activation of Soluble Guanylyl Cyclase. Dev Cell 21(2): 288-300, 2011. View Abstract

Cullum R, Alder O, Hoodless PA. The next generation: using new sequencing technologies to analyze gene regulation. Respirology 16(2):210-222, 2010. View Abstract

Robertson G, Schein J, Chiu R, Corbett R, Field M, Jackman SD, Mungall K, Lee S, Okada HM, Qian JQ, Griffith M, Raymond A, Thiessen N, Cezard T, Butterfield YS, Newsome R, Chan SK, She R, Varhol R, Kamoh B, Prabhu AL, Tam A, Zhao Y, Moore RA, Hirst M, Marra MA, Jones SJ, Hoodless PA, Birol I. De novo assembly and analysis of RNA-seq data. Nat Methods 7(11): 909-912, 2010.  View Abstract

Hoffman BG, Robertson G, Zavaglia PB, Beach M, Cullum R, Lee S, Soukhatcheva G, Li L, Wederell ED, Thiessen N, Bilenky M, Cezard T, Tam A, Kamoh B, Birol I, Dai D, Zhao YJ, Hirst M, Verchere B, Helgason CD, Marra MA, Jones SJM, Hoodless PA. Locus co-occupancy, nucleosome positioning, and H3K4me1 regulate the functionality of FOXA2-, HNF4A-, and PDX1-bound loci in islets and liver. Genome Res 20(8): 1037-51, 2010. View Abstract

Hassan AS, Hou J, Wei W, Hoodless PA, Expression of two novel transcripts in the mouse definitive endoderm. Gene Expr Patterns 10:127-134, 2010. View Abstract

McKnight K, Hou J, Hoodless PA.  FoxH1 and FoxA2 are not required for formation of the midgut and hindgut definitive endoderm.  Dev Biol 337: 471-481, 2010. View Abstract

Vrljicak P, Chang AC, Morozova O, Wederell ED, Niessen K, Marra MA, Karsan A, Hoodless PA. Genomic Analysis Distinguishes Phases of Early Development of the Mouse Atrio-Ventricular Canal. Physiol Genomics 40(3): 150-157, 2010. View Abstract

Wederell ED, Bilenky M , Cullum R, Thiessen N, Dagpinar M, Delaney A, Varhol R, Zhao YJ, Zeng T, Bernier B, Ingham M, Hirst M, Robertson G Marra MA, Jones S, Hoodless PA. Global Analysis of In Vivo FoxA2 Binding Sites in Mouse Adult Liver Using Massively Parallel Sequencing.  Nuc Acids Res 36: 4549-4564, 2008. View Abstract

Robertson AG, Bilenky M, Tam A, Zhao Y, Zeng T, Thiessen N, Cezard T, Fejes A, Wederell E, Cullum R, Euskirchen G, Krzywinski M, Birol I, Snyder M, Hoodless PA, Hirst M, Marra MA, Jones SJM.  Genome wide relationship between histone H3 lysine 4 mono-and-tri methylation and transcription factor binding.  Genome Res 18:1906-1917, 2008 View Abstract

Niessen K, Fu YX, Chang L, Wong F, McFadden D, Hoodless PA & Karsan A. Slug is a direct Notch target required for initiation of cardiac cushion cellularization.  J Cell Biol 182:315-25, 2008.  View Abstract

Hoffman BG, Zavaglia B, Witzsche J, Ruiz de Algara T, Beach M, Hoodless PA, Jones SJ, Marra MA, & Helgason CD. Identification of transcripts with enriched expression in the developing and adult pancreas. Genome Biol 9 (6): R99, 2008. View Article

BowieMB, Kent DG, Dykstra B, McKnight KD, McCaffrey L, Hoodless PA, & Eaves CJ. Identification of a new intrinsically timed developmental checkpoint that reprograms key hematopoietic stem cell properties. Proc Natl Acad Sci U S A 104 (14): 5878-82, 2007. View Abstract

Hou J, Charters AM, Lee SC, Zhao Y, Wu MK, Jones SJ, Marra MA, & Hoodless PA. A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE). BMC Dev Biol 7: 92, 2007. View Abstract

Khattra J, Delaney AD, Zhao Y, Siddiqui A, Asano J, McDonald H, Pandoh P, Dhalla N, Prabhu AL, Ma K, Lee S, Ally A, Tam A, Sa D, Rogers S, Charest D, Stott J, Zuyderduyn S, Varhol R, Eaves C, Jones S, Holt R, Hirst M, Hoodless PA, & Marra MA. Large-scale production of SAGE libraries from microdissected tissues, flow-sorted cells, and cell lines. Genome Res 17 (1): 108-16, 2007. View Abstract

McKnight KD, Hou J, & Hoodless PA. Dynamic expression of thyrotropin-releasing hormone in the mouse definitive endoderm. Dev Dyn 236 (10): 2909-17, 2007. View Abstract

Noorali S, Kurita T, Woolcock B, de Algara TR, Lo M, Paralkar V, Hoodless P, & Vielkind J. Dynamics of expression of growth differentiation factor 15 in normal and PIN development in the mouse. Differentiation 75 (4): 325-36, 2007. View Abstract

Bowie MB, McKnight KD, Kent DG, McCaffrey L, Hoodless PA, & Eaves CJ. Hematopoietic stem cells proliferate until after birth and show a reversible phase-specific engraftment defect. J Clin Invest 116 (10): 2808-16, 2006. View Abstract

Houde C, Dickinson RJ, Houtzager VM, Cullum R, Montpetit R, Metzler M, Simpson EM, Roy S, Hayden MR, Hoodless PA, & Nicholson DW. Hippi is essential for node cilia assembly and Sonic hedgehog signaling. Dev Biol 300 (2): 523-33, 2006. View Abstract

Mar L, & Hoodless PA. Embryonic fibroblasts from mice lacking Tgif were defective in  cell cycling. Mol Cell Biol 26 (11): 4302-10, 2006. View Abstract

Siddiqui AS, Khattra J, Delaney AD, Zhao Y, Astell C, Asano J, Babakaiff R, Barber S, Beland J, Bohacec S, Brown-John M, Chand S, Charest D, Charters AM, Cullum R, Dhalla N, Featherstone R, Gerhard DS, Hoffman B, Holt RA, Hou J, Kuo BY, Lee LL, Lee S, Leung D, Ma K, Matsuo C, Mayo M, McDonald H, Prabhu AL, Pandoh P, Riggins GJ, de Algara TR, Rupert JL, Smailus D, Stott J, Tsai M, Varhol R, Vrljicak P, Wong D, Wu MK, Xie YY, Yang G, Zhang I, Hirst M, Jones SJ, Helgason CD, Simpson EM, Hoodless PA, & Marra MA. A mouse atlas of gene expression: large-scale digital gene-expression profiles from precisely defined developing C57BL/6J mouse tissues and cells. Proc Natl Acad Sci U S A 102 (51): 18485-90, 2005. View Abstract

Noseda M, McLean G, Niessen K, Chang L, Pollet I, Montpetit R, Shahidi R, Dorovini-Zis K, Li L, Beckstead B, Durand RE, Hoodless PA, & Karsan A. Notch activation results in phenotypic and functional changes consistent with endothelial-to-mesenchymal transformation. Circ Res 94 (7): 910-7, 2004. View Abstract

Hoodless PA, Pye M, Chazaud C, Labbe E, Attisano L, Rossant J, & Wrana JL. FoxH1 (Fast) functions to specify the anterior primitive streak in the mouse. Genes Dev 15 (10): 1257-71, 2001. View Abstract

Sirard C, Kim S, Mirtsos C, Tadich P, Hoodless PA, Itie A, Maxson R, Wrana JL, & Mak TW. Targeted disruption in murine cells reveals variable requirement for Smad4 in transforming growth factor beta-related signaling. J Biol Chem 275 (3): 2063-70, 2000. View Abstract

Tremblay KD, Hoodless PA, Bikoff EK, & Robertson EJ. Formation of the definitive endoderm in mouse is a Smad2-dependent process. Development 127 (14): 3079-90, 2000. View Abstract

Hoodless PA, Tsukazaki T, Nishimatsu S, Attisano L, Wrana JL, & Thomsen GH. Dominant-negative Smad2 mutants inhibit activin/Vg1 signaling and disrupt axis formation in Xenopus. Dev Biol 207 (2): 364-79, 1999. View Abstract

Hoodless PA, & Wrana JL. Mechanism and function of signaling by the TGF beta superfamily. Curr Top Microbiol Immunol 228: 235-72, 1998. View Abstract

Labbe E, Silvestri C, Hoodless PA, Wrana JL, & Attisano L. Smad2 and Smad3 positively and negatively regulate TGF beta-dependent transcription through the forkhead DNA-binding protein FAST2. Mol Cell 2 (1): 109-20, 1998. View Abstract

Macias-Silva M, Hoodless PA, Tang SJ, Buchwald M, & Wrana JL. Specific activation of Smad1 signaling pathways by the BMP7 type I receptor, ALK2. J Biol Chem 273 (40): 25628-36, 1998. View Abstract

Tang SJ, Hoodless PA, Lu Z, Breitman ML, McInnes RR, Wrana JL, & Buchwald M. The Tlx-2 homeobox gene is a downstream target of BMP signalling and is required for mouse mesoderm development. Development 125 (10): 1877-87, 1998. View Abstract

Waldrip WR, Bikoff EK, Hoodless PA, Wrana JL, & Robertson EJ. Smad2 signaling in extraembryonic tissues determines anterior-posterior polarity of the early mouse embryo. Cell 92 (6): 797-808, 1998. View Abstract

Hoodless PA, Hemmati-Brivanlou A.  Inhibitory control of neural differentiation in mammalian cells.  Development, Genes and Evolution 207:19-28, 1997. View Abstract

Li M, Li J, Hoodless PA, Tzukazaki T, Wrana JL, Attisano L, & Tsang BK. Mothers against decapentaplegic-related protein 2 expression in avian granulosa cells is up-regulated by transforming growth factor beta during ovarian follicular development. Endocrinology 138 (9): 3659-65, 1997. View Abstract

Eppert K, Scherer SW, Ozcelik H, Pirone R, Hoodless P, Kim H, Tsui LC, Bapat B, Gallinger S, Andrulis IL, Thomsen GH, Wrana JL, & Attisano L. MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma. Cell 86 (4): 543-52, 1996. View Abstract

Hoodless PA, Haerry T, Abdollah S, Stapleton M, O’Connor MB, Attisano L, & Wrana JL. MADR1, a MAD-related protein that functions in BMP2 signaling pathways. Cell 85 (4): 489-500, 1996. View Abstract

Macias-Silva M, Abdollah S, Hoodless PA, Pirone R, Attisano L, & Wrana JL. MADR2 is a substrate of the TGFbeta receptor  and its phosphorylation is required for nuclear accumulation and signaling. Cell 87 (7): 1215-24, 1996. View Abstract

Duncan SA, Manova K, Chen WS, Hoodless P, Weinstein DC, Bachvarova RF, & Darnell JE, Jr. Expression of transcription factor HNF-4 in the extraembryonic endoderm, gut, and nephrogenic tissue of the developing mouse embryo: HNF-4 is a marker for primary endoderm in the implanting blastocyst. Proc Natl Acad Sci U S A 91 (16): 7598-602, 1994. View Abstract

WeinsteinDC, Ruiz i Altaba A, Chen WS, Hoodless P, Prezioso VR, Jessell TM, & Darnell JE, Jr. The winged-helix transcription factor HNF-3 beta is required for notochord development in the mouse embryo. Cell 78 (4): 575-88, 1994. View Abstract

Hoodless PA, Ryan AK, Schrader TJ, & Deeley RG. Characterization of liver-enriched proteins binding to a developmentally demethylated site flanking the avian apoVLDLII gene. DNA Cell Biol 11 (10): 755-65, 1992. View Abstract

Hoodless PA, Roy RN, Ryan AK, Hache RJ, Vasa MZ, & Deeley RG. Developmental regulation of specific protein interactions with an enhancerlike binding site far upstream from the avian very-low-density apolipoprotein II gene. Mol Cell Biol 10 (1): 154-64, 1990. View Abstract


Jordan Cran

Research Projects Manager

Rebecca Cullum

Cancer Research Technologist

Sibyl Drissler

Bioinformatics Technologist

Dr Bettina Fuglerud

Postdoctoral Fellow

Nicole Hofs

Assistant Manager, Transgenic Core

April Tian

Research Student