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Principal Investigators

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Dr. Pamela Hoodless — PhD

Director & Distinguished Scientist

Laboratory Contact

Rebecca Cullum

Cancer Research Technologist
  604.675.8000 Ext. 7724

Admin Contact

Jordan Cran

Research Project Manager
  604-675-8126
Overview
Research Interest
Publications
Lab Members
  • Professor, Medical Genetics, University of British Columbia
  • Professor, School of Biomedical Engineering, University of British Columbia
  • Faculty Member, Genome Science & Technology Graduate Program, University of British Columbia
  • Faculty Member, Developmental and Cell Biology, University of British Columbia
  • Associate Member, Bioinformatics Training Program, CIHR
  • Associate Member, Department of Pathology, University of British Columbia

My laboratory explores regulatory mechanisms in organ development, in particular the liver and heart valves. We are using state of the art genomic technologies to shed new light on developmental gene networks by using whole tissue and single cell analyses of gene expression, transcription factor function and epigenetic mechanisms.

HOW DO WE MAKE A LIVER?

The liver is a fascinating organ which controls a wide-array of homeostatic processes in the body including detoxification of metabolites and chemicals, glucose metabolism, synthesis of lipids, production of serum proteins, and bile production. Many people will experience liver disease in their lifetime but, unlike most tissues, this organ retains the ability to regenerate itself when damaged. Despite this ability, chronic liver disease is on the rise. Obesity is the leading cause of liver disease, with over-exposure to common chemicals or medications and viral infections such as hepatitis, being major contributors. Regardless of the initiating factor, chronic liver disease can cause permanent liver damage and scarring that will progress to cirrhosis, poor liver function and can advance to cancer. Currently, there is no cure for cirrhosis. When it is no longer possible to manage the disease, a liver transplant is required, but demand far exceeds available donor tissue. A full understanding of how a liver develops in the embryo will contribute to better ways of treating disease, as well as the generation of alternative sources for liver transplantation.

Liver formation initiates as an epithelial bud that evaginates from the foregut endoderm in response to signals from neighbouring mesoderm. This bud grows and, in association with mesenchyme, endothelium and blood cells, differentiates to form the functional organ. We seek to understand the regulatory processes in early liver formation, including spatial signalling and cellular differentiation, in both mouse and human pluripotent stem cells.

We have two main questions that we are currently exploring:

What are the mechanisms through which transcription factors and epigenetic modifications cooperate to regulate tissue development?

We are using the mouse and human pluripotent stem cells to study the transcriptional networks that control differentiation. Our studies centre on two transcription factors essential for hepatocyte development and function, Hepatocyte Nuclear Factor 4A and FoxA2. Our work shows that these factors have distinct and overlapping functions in development and in the adult. However, it is unclear how the embryonic and adult specific functions are controlled. We are studying the associated epigenetic mechanisms to better understand how these transcriptions factors function to drive differentiation. By studying how sequence-specific, DNA-binding factors regulate transcriptional networks, interact with histone and DNA modifications, and with the enzymes that catalyze these modifications, we can explore how differentiation steps are controlled in specific ways.

How do cell-cell communications drive regulatory networks required for normal liver formation?

Although the primary functional cells in the liver, hepatocytes and cholangiocytes (bile duct), arise from the endoderm, other types of cells contribute to the development of a normal liver through cell-cell communication including endothelial, mesenchyme and blood cells. Cell-cell communications are important for proper liver development. However, we know very little about their role in hepatocyte and cholangiocyte differentiation or in liver morphology. We are using single cell and genome-wide analysis to explore the diversity of cells in the embryonic liver.

HOW DO WE MAKE HEART VALVES?

Congenital heart malformations are the most common form of birth defects, with many of these involving the heart valves. In the embryonic heart, the valves develop in the region between the atria and the ventricles, known as the atrioventricular canal (AVC) and in the region where blood flows into the major arteries, known as the outflow tract (OFT). Valve formation initiates in these regions when endothelial cells which line of the interior of the heart, undergo endothelial-to-mesenchymal transition (EMT) to form endocardial cushions. The cushions undergo remodeling through cellular differentiation to form the valve leaflets and the valve interstitial cells secrete the extracellular matrix that provides the mechanical strength and flexibility to the valve leaflets. Very little is known about valve remodelling during development and how these processes are altered in valve disease. Understanding development may lead to alternative interventions in valve disease.

How do transcriptional networks drive heart valve formation?

The transcription factor, SOX9, is essential to several aspects of valve development, but it also important in the development of many other tissues. Our work has shown that SOX9 plays a central role in regulating the transcriptional networks that drive valve differentiation. Moreover, SOX9 may function as a pioneer factor to establish gene expression necessary for normal development. We are using SOX9 as a window to understand the transcriptional and epigenetic changes that occur during valve development and how SOX9 functions to regulate distinct embryonic differentiation programs.

Selected Publications

Thakur A, Hoodless PA. Repressive epigenetic signatures safeguard the liver. Dev Cell 50:3-4, 2019. View Abstract

Coulombe P, Paliouras GN, Clayton A, Hussainkhel A, Fuller M, Jovanovic V, Dauphinee S, Umlandt P, Xiang P, Kyle AH, Minchinton AI, Humphries RK, Hoodless PA, Parker JDK, Wright JL, Karsan A. Endothelial Sash1 Is Required for Lung Maturation through Nitric Oxide Signaling. Cell Reports. 27:1769-1780, 2019. View Abstract

Nowotschin S, Setty M, Kuo YY, Liu V, Garg V, Sharma R, Simon CS, Saiz N, Gardner R, Boutet SC, Church DM, Hoodless PA, Hadjantonakis AK, Pe'er D. The emergent landscape of the mouse gut endoderm at single-cell resolution. Nature. 569:361-367. 2019  View Abstract

Thakur A, Wong JCH, Wang EY, Lotto J, Kim D, Cheng JC, Mingay M, Cullum R, Moudgil V, Ahmed N, Tsai SH, Wei W, Walsh CP, Stephan T, Bilenky M, Fuglerud BM, Karimi MM, Gonzalez FJ, Hirst M, Hoodless PA. HNF4A is essential for the active epigenetic state at enhancers in mouse liver. Hepatology. 2019 Apr 1. View Abstract [Epub ahead of print]

Wang EY, Cheng J-C, Thakur A,Yi Y, Tsai S-H, Hoodless PA. YAP transcriptionally regulates ErbB2 to promote liver cell proliferation. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms. 1861(9): 854-863, 2018. View Abstract

Cheng J-C, Wang EY, Yi Y, Thakur A, Tsai S-H, Hoodless PA. S1P Stimulates Proliferation by Up-regulating CTGF Expression through S1PR2-mediated YAP Activation. Molecular Cancer Research. 16(10): 1543-1555, 2018. View Abstract

Wei W, Lotto J, Hoodless PA. Expression patterns of Yes-associated protein 1 in the developing mouse liver. Gene Expr Patterns. 29: 10-17, 2018. View Abstract

Xiang P, Wei W, Hoff N, Clemans-Gibbon J, Maetzig T, Lai C, Dhillon I, May C, Rushmann J, Rosten P, Hu K, Hoodless PA, Humphries RK. A knock-in Meis1 mouse strain facilitates dynamic tracking and enrichment of Meis1. Blood Adv. 24(1): 2225-2235, 2017. View Abstract

Yi Y, Tsai S-H, Cheng J-C, Wang EY, Anglesio MS, Cochrane DR, Fuller M, Gibb EA, Wei W, Huntsman DG, Karsan A, Hoodless PA. APELA promotes tumour growth and cell migration in ovarian cancer in a p53-dependent manner. Gynecologic Oncology. 147(3): 663-671, 2017. View Abstract

Freyer L, Hsu C-W, Nowotschin S, Pauli A, Ishida J, Kuba K, Fukamizu A, Schier AF, Hoodless PA, Dickinson ME, Hadjantonakis A-K. Loss of Apela peptide in mice causes low penetrance embryonic lethality and defects in early mesodermal derivatives. Cell Reports. 20(9):2116-2130, 2017. View Abstract

Wilcox SM, Arora H, Munro L, Xin J, Fenninger F, Johnson LA, Pfiefer CG, Choi KB, Hou J, Hoodless PA, Jefferies WA. The Role of the Innate Immune Response Regulatory Gene ABCF1 in Mammalian Embryogenesis and Development.  PlosOne.  12(5):e0175918, 2017. View Abstract

Garside VC, Cullum R, Alder O, Lu DY, Vander Werff R, Bilenky M, Zhao Y, Jones SJM, Marra MA, Underhill TM, Hoodless PA. SOX9 modulates the expression of key transcription factors required for heart valve development. Development. 142: 4340-4350, 2015. View Abstract

Pon J, Wong J, Saberi S, Alder O, Moksa M, Cheng SWG, Morin G, Hoodless PA, Hirst M & Marra, M. MEF2B mutations in non-Hodgkin lymphoma dysregulate cell migration by decreasing MEF2B target gene activation. Nat Commun. 6:7953. 2015 View Abstract

Sanders SS, Hou J, Sutton LM, Garside, VC, Mui KK, Singaraja RR, Hayden MR & Hoodless PA. Huntingtin interacting protein 14 and 14-like are required for chorioallantoic fusion during early placental development. Dev Biol. 397(2):257-66, 2015. View Abstract

Alder O, Cullum R, Lee S, Kan AC, Wei W, Yi Y, Garside VC, Bilenky M, Griffiths M, Morrissy AS, , Robertson GA, Thiessen N, Zhao YJ, Chen Q, Pan D, Jones SJM, Marra MA, Hoodless PA. Hippo signalling influences HNF4A and FOXA2 enhancer switching during hepatocyte differentiation. Cell Rep. 9(1):261-71, 2014.  View Abstract

Hou J, Wei W, Saund RS, Xiang P, Cunningham TJ, Yi Y, Lu DYD, Savory JGA, Krentz NAJ, Montpetit R, Cullum R, Lohnes D, Humphries RK, Yamanaka Y, Duester G, Saijoh Y, Hoodless PA. A regulatory network controls Nephrocan expression and midgut patterning.  Development. 141(19):3772-81, 2014. View Abstract

Chang ACY, Garside VC, Fournier M, Smrz J, Vrljicak P, Umlandt P, Fuller M, Robertson G, Zhao Y, Tam A, Jones SJ, Marra MA, Hoodless PA, Karsan A. A Notch-dependent transcriptional hierarchy promotes mesenchymal transdifferentiation in the cardiac cushion. Dev Dyn. 243:894-905, 2014 View Abstract

Ye Y, Zhang HF, Qiu Y, Hanson PJ, Hemida MJ, Wei W, Hoodless P, Chu F & Yang D. Coxsackievirus-induced miR-21 disrupts cardiomyocyte interactions via the downregulation of intercalated disk components. PLoS Pathog 10: e1004070, 2014. View Abstract

Xiang P, Wei W, Lo C, Rosten P, Hou J, Hoodless PA, Bilenky M, Bonifer C, Cockeril PN, Kirkpatrick A, Gottgens B, Hirst M, Humphries KR. Delineating MEIS1 cis-regulatory elements active in hematopoietic cells. Leukemia, 28:433–436, 2014. View Abstract

Ye X, Zhang HM, Qiu Y, Hanson PJ, Hemida MG, Wei W, Hoodless PA, Chu F, Yang D. Coxsackievirus-induced miR-21 disrupts cardiomyocyte interactions via the downregulation of intercalated disk components. PLoS Pathog. 10:e1004070, 2014 View Abstract

Swanson L, Robertson G, Mungall KL, Butterfield YS, Chiu R, Corbett RD, Docking TR, Hogge D, Jackman SD, Moore RA, Mungall AJ, Nip KM, Parker JD, Qian JQ, Raymond A, Sung S, Tam A, Thiessen N, Varhol R, Wang S, Yorukoglu D, Zhao Y, Hoodless PA, Sahinalp SC, Karsan A, Birol I. Barnacle: detecting and characterizing tandem duplications and fusions in transcriptome assemblies. BMC Genomics 14:550, 2013 View Abstract

Wei W, Hou J, Alder O, Ye X, Lee S, Cullum R, Chu A, Zhao Y, Warner SM, Knight DA, Yang D, Jones SJ, Marra MA, Hoodless PA. Genome-wide microRNA and mRNA profiling in mouse liver development implicates mir302b and mir20a in repressing TGFβ signaling. Hepatology 57:2491-501,2013. View Abstract

Tennant BR, Robertson G, Kramer M, Li L, Zhang X, Beach M, Thiessen N, Chiu R, Mungall K, Whiting CJ, Sabatini P, Kim A, Gottardo R, Marra MA, Lynn F, Jones SJM, Hoodless PA, Hoffman BG. Identification and analysis of murine pancreatic islet enhancers. Diabetologica 56: 542-52, 2013. View Abstract

Garside VC, Chang ACY, Karsan A, Hoodless PA. Co-ordinating Notch, BMP, and TGFβ signalling during heart valve development. Cell Mol Life Sci [epub ahead of print Nov 16], 2012. Invited Review View Abstract

Vrljicak P, CullumR, Xu E, Chang ACY, Wederell ED, Bilenky M, Jones SJM, Marra MA, Karsan A, Hoodless PA. Twist1 transcriptional targets in the developing atrio-ventricular canal of the mouse. PLoS One 7:e40815, 2012. View Abstract

Hneio M, Blirando K, Buard V, Tarlet G, Benderitter, Hoodless PA, Francois A, Milliat F. The TG-interacting factor TGIF1 regulates stress-induced pro-inflammatory phenotype of endothelial cells. J Biol Chem 287: 38913-21, 2012. View Abstract

Hitz MP, Lemieux-Perrault LP, Marshall CR, ZadaYF, Davies R, Yang SW, Lionel AC, D’Amours G, Lemyre E, Cullum R, Bigras JL, Thibeault M, Chetaille P, Montpetit A, Khairy P, Overduin B, Klaassen S, Hoodless P, Nemer M, Stewart AFR, Boerkoel C, Scherer SW, Richter A, Dube MP, Andelfinger G. Rare copy number variants contribute to congenital left-sided heart disease. PLoS Genet 8: e1002903, 2012. View Abstract

Hneino M, Francois A, Buard V, Tarlet G, Abderrahmani R, Blirando K, Hoodless PA, Benderitter M, Milliat F. The TGF-b/Smad repressor TG-interacting factor 1 (TGIF1) plays a role in radiation-induced-intestinal injury independently of a Smad signaling pathway. PLoS One 7: e35672, 2012. View Abstract

Yusuf D, Butland SL, Swanson MI, Bolotin E, Ticoll A, Cheung WA, Yu XY, Zhang C, Dickman CTD, Fulton DL, Lim JS, Schnabl JM, Ramos OHP, Vasseur-Cognet M, de Leeuw CN, Simpson EM, Ryffel GU, Lam EWF, Kist R, Wilson MSC, Marco-Ferreres R, Brosens JJ, Beccari LL, Bovolenta P, Benayoun BA, Monteiro LJ, Schwenen HDC, Grontved L, Wederell E, Mandrup S, Veitia RA, Chakravarthy H, Hoodless PA, Mancarelli MM, Torbett BE, Banham AH, Reddy SP, Cullum RL, Liedtke M, Tschan MP, Vaz M, Rizzino A, Zannini M, Frietze S, Farnham PJ, Eijkelenboom A, Brown PJ, Laperrière D, Leprince D, de Cristofaro T, Prince KL, Putker M, del Peso L, Camenisch G, Wenger RH, Mikula M, Rozendaal M, Mader S, Ostrowski J, Rhodes SJ, Van Rechem C, Boulay G, Olechnowicz SWZ, Breslin MB, Lan MS, Nanan KK, Wegner M, Hou J, Mullen RD, Colvin SC, Noy PJ, Webb CF, Witek ME, Ferrell S, Daniel JM, Park J, Waldman SA,  Peet DJ, Taggart M, Jayaraman P-S, Karrich JJ, Blom B, Vesuna F, O'Geen H, Sun Y, Gronostajski RM, Woodcroft MW, Hough MR, Chen E, Europe-Finner GN, Karolczak-Bayatti M, Bailey J, Hankinson O, Raman V, LeBrun DP, Biswal S, Harvey CJ, DeBruyne JP, Hogenesch JB, Hevner RF, Héligon C, Luo XM, Blank MC, Millen KJ, Sharlin DS, Forrest D, Dahlman-Wright K, Zhao C, Mishima Y, Sinha S, Chakrabarti R, Portales-Casamar E, Sladek FM, Bradley PH and Wasserman WW. The Transcription Factor Encyclopedia. Genome Biol 13:R24, 2012. View Abstract

Chang AC, Fu Y, Garside VC, Niessen K, Chang L, Fuller M, Setiadi A, Smrz J, Kyle A, Minchinton A, Marra M, Hoodless PA, Karsan A. Notch Initiates the Endothelial-to-Mesenchymal Transition in the Atrioventricular Canal through Autocrine Activation of Soluble Guanylyl Cyclase. Dev Cell 21(2): 288-300, 2011. View Abstract

Cullum R, Alder O, Hoodless PA. The next generation: using new sequencing technologies to analyze gene regulation. Respirology 16(2):210-222, 2010. View Abstract

Robertson G, Schein J, Chiu R, Corbett R, Field M, Jackman SD, Mungall K, Lee S, Okada HM, Qian JQ, Griffith M, Raymond A, Thiessen N, Cezard T, Butterfield YS, Newsome R, Chan SK, She R, Varhol R, Kamoh B, Prabhu AL, Tam A, Zhao Y, Moore RA, Hirst M, Marra MA, Jones SJ, Hoodless PA, Birol I. De novo assembly and analysis of RNA-seq data. Nat Methods 7(11): 909-912, 2010.  View Abstract

Hoffman BG, Robertson G, Zavaglia PB, Beach M, Cullum R, Lee S, Soukhatcheva G, Li L, Wederell ED, Thiessen N, Bilenky M, Cezard T, Tam A, Kamoh B, Birol I, Dai D, Zhao YJ, Hirst M, Verchere B, Helgason CD, Marra MA, Jones SJM, Hoodless PA. Locus co-occupancy, nucleosome positioning, and H3K4me1 regulate the functionality of FOXA2-, HNF4A-, and PDX1-bound loci in islets and liver. Genome Res 20(8): 1037-51, 2010. View Abstract

Hassan AS, Hou J, Wei W, Hoodless PA, Expression of two novel transcripts in the mouse definitive endoderm. Gene Expr Patterns 10:127-134, 2010. View Abstract

McKnight K, Hou J, Hoodless PA.  FoxH1 and FoxA2 are not required for formation of the midgut and hindgut definitive endoderm.  Dev Biol 337: 471-481, 2010. View Abstract

Vrljicak P, Chang AC, Morozova O, Wederell ED, Niessen K, Marra MA, Karsan A, Hoodless PA. Genomic Analysis Distinguishes Phases of Early Development of the Mouse Atrio-Ventricular Canal. Physiol Genomics 40(3): 150-157, 2010. View Abstract

Wederell ED, Bilenky M , Cullum R, Thiessen N, Dagpinar M, Delaney A, Varhol R, Zhao YJ, Zeng T, Bernier B, Ingham M, Hirst M, Robertson G Marra MA, Jones S, Hoodless PA. Global Analysis of In Vivo FoxA2 Binding Sites in Mouse Adult Liver Using Massively Parallel Sequencing.  Nuc Acids Res 36: 4549-4564, 2008. View Abstract

Robertson AG, Bilenky M, Tam A, Zhao Y, Zeng T, Thiessen N, Cezard T, Fejes A, Wederell E, Cullum R, Euskirchen G, Krzywinski M, Birol I, Snyder M, Hoodless PA, Hirst M, Marra MA, Jones SJM.  Genome wide relationship between histone H3 lysine 4 mono-and-tri methylation and transcription factor binding.  Genome Res 18:1906-1917, 2008 View Abstract

Niessen K, Fu YX, Chang L, Wong F, McFadden D, Hoodless PA & Karsan A. Slug is a direct Notch target required for initiation of cardiac cushion cellularization.  J Cell Biol 182:315-25, 2008.  View Abstract

Hoffman BG, Zavaglia B, Witzsche J, Ruiz de Algara T, Beach M, Hoodless PA, Jones SJ, Marra MA, & Helgason CD. Identification of transcripts with enriched expression in the developing and adult pancreas. Genome Biol 9 (6): R99, 2008. View Article

BowieMB, Kent DG, Dykstra B, McKnight KD, McCaffrey L, Hoodless PA, & Eaves CJ. Identification of a new intrinsically timed developmental checkpoint that reprograms key hematopoietic stem cell properties. Proc Natl Acad Sci U S A 104 (14): 5878-82, 2007. View Abstract

Hou J, Charters AM, Lee SC, Zhao Y, Wu MK, Jones SJ, Marra MA, & Hoodless PA. A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE). BMC Dev Biol 7: 92, 2007. View Abstract

Khattra J, Delaney AD, Zhao Y, Siddiqui A, Asano J, McDonald H, Pandoh P, Dhalla N, Prabhu AL, Ma K, Lee S, Ally A, Tam A, Sa D, Rogers S, Charest D, Stott J, Zuyderduyn S, Varhol R, Eaves C, Jones S, Holt R, Hirst M, Hoodless PA, & Marra MA. Large-scale production of SAGE libraries from microdissected tissues, flow-sorted cells, and cell lines. Genome Res 17 (1): 108-16, 2007. View Abstract

McKnight KD, Hou J, & Hoodless PA. Dynamic expression of thyrotropin-releasing hormone in the mouse definitive endoderm. Dev Dyn 236 (10): 2909-17, 2007. View Abstract

Noorali S, Kurita T, Woolcock B, de Algara TR, Lo M, Paralkar V, Hoodless P, & Vielkind J. Dynamics of expression of growth differentiation factor 15 in normal and PIN development in the mouse. Differentiation 75 (4): 325-36, 2007. View Abstract

Bowie MB, McKnight KD, Kent DG, McCaffrey L, Hoodless PA, & Eaves CJ. Hematopoietic stem cells proliferate until after birth and show a reversible phase-specific engraftment defect. J Clin Invest 116 (10): 2808-16, 2006. View Abstract

Houde C, Dickinson RJ, Houtzager VM, Cullum R, Montpetit R, Metzler M, Simpson EM, Roy S, Hayden MR, Hoodless PA, & Nicholson DW. Hippi is essential for node cilia assembly and Sonic hedgehog signaling. Dev Biol 300 (2): 523-33, 2006. View Abstract

Mar L, & Hoodless PA. Embryonic fibroblasts from mice lacking Tgif were defective in  cell cycling. Mol Cell Biol 26 (11): 4302-10, 2006. View Abstract

Siddiqui AS, Khattra J, Delaney AD, Zhao Y, Astell C, Asano J, Babakaiff R, Barber S, Beland J, Bohacec S, Brown-John M, Chand S, Charest D, Charters AM, Cullum R, Dhalla N, Featherstone R, Gerhard DS, Hoffman B, Holt RA, Hou J, Kuo BY, Lee LL, Lee S, Leung D, Ma K, Matsuo C, Mayo M, McDonald H, Prabhu AL, Pandoh P, Riggins GJ, de Algara TR, Rupert JL, Smailus D, Stott J, Tsai M, Varhol R, Vrljicak P, Wong D, Wu MK, Xie YY, Yang G, Zhang I, Hirst M, Jones SJ, Helgason CD, Simpson EM, Hoodless PA, & Marra MA. A mouse atlas of gene expression: large-scale digital gene-expression profiles from precisely defined developing C57BL/6J mouse tissues and cells. Proc Natl Acad Sci U S A 102 (51): 18485-90, 2005. View Abstract

Noseda M, McLean G, Niessen K, Chang L, Pollet I, Montpetit R, Shahidi R, Dorovini-Zis K, Li L, Beckstead B, Durand RE, Hoodless PA, & Karsan A. Notch activation results in phenotypic and functional changes consistent with endothelial-to-mesenchymal transformation. Circ Res 94 (7): 910-7, 2004. View Abstract

Hoodless PA, Pye M, Chazaud C, Labbe E, Attisano L, Rossant J, & Wrana JL. FoxH1 (Fast) functions to specify the anterior primitive streak in the mouse. Genes Dev 15 (10): 1257-71, 2001. View Abstract

Sirard C, Kim S, Mirtsos C, Tadich P, Hoodless PA, Itie A, Maxson R, Wrana JL, & Mak TW. Targeted disruption in murine cells reveals variable requirement for Smad4 in transforming growth factor beta-related signaling. J Biol Chem 275 (3): 2063-70, 2000. View Abstract

Tremblay KD, Hoodless PA, Bikoff EK, & Robertson EJ. Formation of the definitive endoderm in mouse is a Smad2-dependent process. Development 127 (14): 3079-90, 2000. View Abstract

Hoodless PA, Tsukazaki T, Nishimatsu S, Attisano L, Wrana JL, & Thomsen GH. Dominant-negative Smad2 mutants inhibit activin/Vg1 signaling and disrupt axis formation in Xenopus. Dev Biol 207 (2): 364-79, 1999. View Abstract

Hoodless PA, & Wrana JL. Mechanism and function of signaling by the TGF beta superfamily. Curr Top Microbiol Immunol 228: 235-72, 1998. View Abstract

Labbe E, Silvestri C, Hoodless PA, Wrana JL, & Attisano L. Smad2 and Smad3 positively and negatively regulate TGF beta-dependent transcription through the forkhead DNA-binding protein FAST2. Mol Cell 2 (1): 109-20, 1998. View Abstract

Macias-Silva M, Hoodless PA, Tang SJ, Buchwald M, & Wrana JL. Specific activation of Smad1 signaling pathways by the BMP7 type I receptor, ALK2. J Biol Chem 273 (40): 25628-36, 1998. View Abstract

Tang SJ, Hoodless PA, Lu Z, Breitman ML, McInnes RR, Wrana JL, & Buchwald M. The Tlx-2 homeobox gene is a downstream target of BMP signalling and is required for mouse mesoderm development. Development 125 (10): 1877-87, 1998. View Abstract

Waldrip WR, Bikoff EK, Hoodless PA, Wrana JL, & Robertson EJ. Smad2 signaling in extraembryonic tissues determines anterior-posterior polarity of the early mouse embryo. Cell 92 (6): 797-808, 1998. View Abstract

Hoodless PA, Hemmati-Brivanlou A.  Inhibitory control of neural differentiation in mammalian cells.  Development, Genes and Evolution 207:19-28, 1997. View Abstract

Li M, Li J, Hoodless PA, Tzukazaki T, Wrana JL, Attisano L, & Tsang BK. Mothers against decapentaplegic-related protein 2 expression in avian granulosa cells is up-regulated by transforming growth factor beta during ovarian follicular development. Endocrinology 138 (9): 3659-65, 1997. View Abstract

Eppert K, Scherer SW, Ozcelik H, Pirone R, Hoodless P, Kim H, Tsui LC, Bapat B, Gallinger S, Andrulis IL, Thomsen GH, Wrana JL, & Attisano L. MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma. Cell 86 (4): 543-52, 1996. View Abstract

Hoodless PA, Haerry T, Abdollah S, Stapleton M, O’Connor MB, Attisano L, & Wrana JL. MADR1, a MAD-related protein that functions in BMP2 signaling pathways. Cell 85 (4): 489-500, 1996. View Abstract

Macias-Silva M, Abdollah S, Hoodless PA, Pirone R, Attisano L, & Wrana JL. MADR2 is a substrate of the TGFbeta receptor  and its phosphorylation is required for nuclear accumulation and signaling. Cell 87 (7): 1215-24, 1996. View Abstract

Duncan SA, Manova K, Chen WS, Hoodless P, Weinstein DC, Bachvarova RF, & Darnell JE, Jr. Expression of transcription factor HNF-4 in the extraembryonic endoderm, gut, and nephrogenic tissue of the developing mouse embryo: HNF-4 is a marker for primary endoderm in the implanting blastocyst. Proc Natl Acad Sci U S A 91 (16): 7598-602, 1994. View Abstract

WeinsteinDC, Ruiz i Altaba A, Chen WS, Hoodless P, Prezioso VR, Jessell TM, & Darnell JE, Jr. The winged-helix transcription factor HNF-3 beta is required for notochord development in the mouse embryo. Cell 78 (4): 575-88, 1994. View Abstract

Hoodless PA, Ryan AK, Schrader TJ, & Deeley RG. Characterization of liver-enriched proteins binding to a developmentally demethylated site flanking the avian apoVLDLII gene. DNA Cell Biol 11 (10): 755-65, 1992. View Abstract

Hoodless PA, Roy RN, Ryan AK, Hache RJ, Vasa MZ, & Deeley RG. Developmental regulation of specific protein interactions with an enhancerlike binding site far upstream from the avian very-low-density apolipoprotein II gene. Mol Cell Biol 10 (1): 154-64, 1990. View Abstract

group-shot-1

Jordan Cran

MSc, PMP
Research Project Manager

Rebecca Cullum

BSc
Cancer Research Technologist

Dr Bettina Fuglerud

PhD
Postdoctoral Fellow

Nicole Hofs

BSc
Assistant Manager, Transgenic Core