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Dr Peter Lansdorp — MD, PhD, FRS(C)

Distinguished Scientist

Overview
Research Interest
Publications
Lab Members

Education:
MD, Erasmus University Rotterdam, 1976
PhD, Experimental Hematology, University of Amsterdam, 1985

  • DNA replication, epigenetics and stem cells
  • Telomeres, genomic instability, aging and cancer
  • Genetic analysis using single cell Strand-seq

Asymmetric cell divisions are essential for the development of all multicellular organisms. Many mechanisms are known to help secure differences in cell fate between daughter cells following the division of a parental stem cell. We are interested in possibility that programmed epigenetic differences between sister chromatids contribute to gene expression differences during development [1]. Such differences may arise when the deposition of parental nucleosomes onto replicated DNA following replication is asymmetric because replication of one of the DNA template strands is delayed because the replisome encounters a problem on one of the template strands. Strand-specific DNA replication problems are not theoretical. We showed in 2002 that the replication of guanine-rich DNA requires a specialized helicase, which we called Dog-1 for Deletion of guanine-rich DNA [2]. We subsequently identified RTEL1 in the mouse as a major regulator of telomere length [3]. DOG-1 (aka FANCJ) and RTEL1 are iron-sulfur cluster helicase proteins that can unwind secondary structures of guanine-rich DNA called guanine quadruplex (G4) structures [4]. G4 structures were recently shown to regulate gene expression [5]. The presence and role of G4 structures at specific genomic locations as well as the enzymes required for resolving G4 structures in genome stability, gene expression and differentiation are areas of special interest. For such studies we recently developed techniques to identify and study sister chromatids in single cells [6, 7]. Current studies in the Lansdorp lab focus on three questions that can only (!) be answered using our single cell sequencing technique called Strand-seq [8]:

1)     Investigate the molecular mechanisms that regulate stem cell self-renewal and differentiation. By combining single cell Strand-seq with the analysis of transcripts in the same cell we are addressing an aspect of stem cell biology that has not been subject to experimentation before.

2)     Elucidate molecular mechanisms involved in DNA repair and epigenetic asymmetry between sister chromatids. By mapping the location of sister chromatid exchange events [9], genome copy number variations [10], polymorphic inversions [11] and loss of heterozygosity (LOH) events [12], we have a unique capability to study DNA events at the level of single cells.  Studies with cells before and after specific genetic alterations are providing new insight in cancer, specific diseases and aging.

3)     Map polymorphic inversions in human chromosomes [11] and combine this information with haplotype information [12] to generate completely phased “personalized genomes” for “precision medicine”. Ongoing studies have revealed that Strand-seq by itself, but especially in combination with other sequencing approaches, yields unprecedented precise information about individual genomes. Apart from proof of principle studies we are exploring better ways to increase the throughput and decrease the cost of our methods.

Reference: 

  1. Lansdorp, P.M., Immortal strands? Give me a break. Cell, 2007. 129(7): p. 1244-7.
  2. Cheung, I., et al., Disruption of dog-1 in Caenorhabditis elegans triggers deletions upstream of guanine-rich DNA. Nat Genet, 2002. 31(4): p. 405-9.
  3. Ding, H., et al., Regulation of murine telomere length by Rtel: an essential gene encoding a helicase-like protein. Cell, 2004. 117(7): p. 873-86.
  4. Bharti, S.K., et al., Specialization among iron-sulfur cluster helicases to resolve G-quadruplex DNA structures that threaten genomic stability. J Biol Chem, 2013. 288(39): p. 28217-29.
  5. Papadopoulou, C., et al., Nucleotide Pool Depletion Induces G-Quadruplex-Dependent Perturbation of Gene Expression. Cell Rep, 2015. 13(11): p. 2491-503.
  6. Falconer, E., et al., Identification of sister chromatids by DNA template strand sequences. Nature, 2010. 463(7277): p. 93-7.
  7. Falconer, E., et al., DNA template strand sequencing of single-cells maps genomic rearrangements at high resolution. Nat Methods, 2012. 9(11): p. 1107-12.
  8. Falconer, E. and P.M. Lansdorp, Strand-seq: a unifying tool for studies of chromosome segregation. Semin Cell Dev Biol, 2013. 24(8-9): p. 643-52.
  9. van Wietmarschen, N. and P.M. Lansdorp, Bromodeoxyuridine does not contribute to sister chromatid exchange events in normal or Bloom syndrome cells. Nucleic Acids Res, 2016. 44(14): p. 6787-93.
  10. Bakker, B., et al., Single-cell sequencing reveals karyotype heterogeneity in murine and human malignancies. Genome Biol, 2016. 17(1): p. 115.
  11. Sanders, A.D., et al., Characterizing polymorphic inversions in human genomes by single-cell sequencing. Genome Res, 2016. 26(11): p. 1575-1587.
  12. Porubsky, D., et al., Direct chromosome-length haplotyping by single-cell sequencing. Genome Res, 2016. 26(11): p. 1565-1574.

 

Porubsky D, Sanders AD, van Wietmarschen N, Falconer E, Hills M, Spierings DC, Bevova MR, Guryev V & Lansdorp PM. Direct chromosome-length haplotyping by single-cell sequencing. Genome Res 26: 1565-1574, 2016. View Abstract

Sanders AD, Hills M, Porubský D, Guryev V, Falconer E, & Lansdorp PM. Characterizing polymorphic inversions in human genomes by single cell sequencing. Genome Res 26: 1575-1587, 2016. View Abstract

van Wietmarschen N & Lansdorp PM. Bromodeoxyuridine does not contribute to sister chromatid exchange events in normal or Bloom syndrome cells. Nucleic Acids Res 44: 6787-93, 2016. View Abstract

van den Bos H, Spierings DC, Taudt AS, Bakker B, Porubský D, Falconer E, Novoa C, Halsema N, Kazemier HG, Hoekstra-Wakker K, Guryev V, den Dunnen WF, Foijer F, Tatché MC, Boddeke HW, & Lansdorp PM. Single-cell whole genome sequencing reveals no evidence for common aneuploidy in normal and Alzheimer's disease neurons. Genome Biol 17: 116, 2016. View Abstract

Lansdorp PM. Editorial: Telomeres on steroids: turning back the mitotic clock? N Engl J Med 374: 1978-80, 2016. PubMed Link

Hoffmann RF, Moshkin YM, Mouton S, Grzeschik NA, Kalicharan RD, Kuipers J, Wolters AH, Nishida K, Romashchenko AV, Postberg J, Lipps H, Berezikov E, Sibon OC, Giepmans BN & Lansdorp PM. Guanine quadruplex structures localize to heterochromatin.  Nucleic Acids Res 44: 152-163, 2016. View Abstract

Benchetrit H, Herman S, van Wietmarschen N, Wu T, Makedonski K, Maoz N, Yom Tov N, Stave D, Lasry R, Zayat V, Xiao A, Lansdorp PM, Sebban S & Buganim Y. Extensive Nuclear Reprogramming Underlies Lineage Conversion into Functional Trophoblast Stem-like Cells.  Cell Stem Cell 17: 543-556, 2015. View Abstract

Bakker B, van den Bos H, Lansdorp PM, Foijer F. How to count chromosomes in a cell: An overview of current and novel technologiesBioessays. 2015 May;37(5):570-7. doi: 10.1002/bies.201400218. View Abstract

Buganim Y, Markoulaki S, van Wietmarschen N, Hoke H, Wu T, Ganz K, Akhtar-Zaidi B, He Y, Abraham BJ, Porubsky D, Kulenkampff E, Faddah DA, Shi L, Gao Q, Sarkar S, Cohen M, Goldmann J, Nery JR, Schultz MD, Ecker JR, Xiao A, Young RA, Lansdorp PM, Jaenisch R. The developmental potential of iPSCs is greatly influenced by reprogramming factor selection. Cell Stem Cell. 2014 Sep 4;15(3):295-309. doi: 10.1016/j.stem.2014. View Abstract

Lisaingo K, Uringa EJ, Lansdorp PM. Resolution of telomere associations by TRF1 cleavage in mouse embryonic stem cells. Mol Biol Cell. 2014 Jul 1;25(13):1958-68. doi: 10.1091/mbc.E13-10-0564. Epub 2014. View Abstract

Gardner M, Bann D, Wiley L, Cooper R, Hardy R, Nitsch D, Martin-Ruiz C, Shiels P, Sayer AA, Barbieri M, Bekaert S, Bischoff C, Brooks-Wilson A, Chen W, Cooper C, Christensen K, De Meyer T, Deary I, Der G, Diez Roux A, Fitzpatrick A, Hajat A, Halaschek-Wiener J, Harris S, Hunt SC, Jaggar C, Jeon HS, Kaplan R, Kimura M, Lansdorp P, Li C, Maeda T, Mangino M, Nawrot TS, Nilsson P, Nordfjall K, Paolisso G, Ren F, Riabowol K, Robertson T, Roos G, Staessen JA, Spector T, Tang N, Unryn B, van der Harst P, Woo J, Xing C, Yadegarfar ME, Park JY, Young N, Kuh D, von Zglinicki T, Ben-Shlomo Y & Halcyon study team. Gender and telomere length: Systematic review and meta-analysis.  Exp Gerontol 51: 15-27, 2014. View Abstract

Henderson A, Wu Y, Huang YC, Chavez EA, Platt J, Johnson FB, Brosh RM, Jr., Sen D & Lansdorp PM. Detection of G-quadruplex DNA in mammalian cells.  Nucleic Acids Res 42: 860-869, 2014. View Abstract

Falconer E & Lansdorp PM. Strand-seq: A unifying tool for studies of chromosome segregation. Semin Cell Dev Biol 24: 643-652, 2013. View Abstract

Hills M, O'Neil K, Falconer E, Brinkman R & Lansdorp PM. BAIT:Organizing genomes and mapping rearrangements in single cells.  Genome Med 5: 82 2013. View Abstract

Kannan N, Huda N, Tu L, Droumeva R, Aubert G, Chavez E, Brinkman RR, Lansdorp P, Emerman J, Abe S, Eaves C & Gilley D. The luminal progenitor compartment of the normal human mammary gland constitutes a unique site of telomere dysfunction.  Stem Cell Reports 1: 28-37, 2013. View Abstract

Falconer E, Hills M, Naumann U, Poon SS, Chavez EA, Sanders AD, Hirst M & Lansdorp PM. DNA template strand sequencing of single-cells maps genomic rearrangements at high resolution.  Nature Methods 9: 1107-1112, 2012. View Abstract

van Wietmarschen N, Moradian A, Morin GB, Lansdorp PM & Uringa EJ. The mammalian proteins MMS19, MIP18, and ANT2 are involved in cytoplasmic iron-sulfur cluster protein assembly.  J Biol Chem 287: 43351-43358, 2012. View Abstract

Frumento G, Zheng Y, Aubert G, Raeiszadeh M, Lansdorp PM, Moss P, Lee SP & Chen FE. Cord blood T cells retain early differentiation phenotype suitable for immunotherapy after TCR gene transfer to confer EBV specificity.  Am J Transplant 13: 45-55, 2012. View Abstract

Lansdorp PM, Falconer E, Tao J, Brind'Amour J & Naumann R. Epigenetic differences between sister chromatids.  Ann N Y Acad Sci 1266: 1-6, 2012. View Abstract

Uringa E-J, Lisaingo K, Pickett HA, Brind'Amour J, Rohde J-H, Zelensky A, Essers J & Lansdorp PM. RTEL1 contributes to DNA replication, repair and telomere maintenance. Mol Biol Cell 23: 2782-92, 2012. View Abstract

Aubert G, Baerlocher GM, Vulto I, Poon SS & Lansdorp PM. Collapse of telomere homeostasis in hematopoietic stem cells caused by heterozygous mutations in telomerase genes. PLoS Genet 8: e1002696, 2012. View Paper

Alter BP, Rosenberg PS, Giri N, Lansdorp PM & Savage SA. Telomere length is associated with disease severity and declines with age in dyskeratosis congenita. Haematologica 97: 353-539, 2012. View Abstract

Sekulovic S, Gylfadottir V, Vulto I, Gasparetto M, Even Y, Brookes C, Smith C, Eaves CJ, Lansdorp PM, Rossi FM & Humphries RK. Prolonged self-renewal activity unmasks telomerase control of telomere homeostasis and function of mouse hematopoietic stem cells. Blood 118: 1766-73, 2011. View Abstract

Aubert G, Hills M & Lansdorp PM. Telomere length measurement-Caveats and a critical assessment of the available technologies and tools. Mutat Res 730: 59-67, 2012. View Abstract

Brind'Amour J & Lansdorp PM. Analysis of repetitive DNA in chromosomes by flow cytometry.  Nat Methods 8: 484-486, 2011. View Abstract

Alder JK, Cogan JD, Brown AF, Anderson CJ, Lawson WE, Lansdorp PM, Philips JA, Loyd JE, Chen JJ & Armanios MY. Ancestral mutation in telomerase causes defects in repeat addition processivity and manifests as familial pulmonary fibrosis.  PLoS Genet 7: e1001352 2011. View Abstract

Shlush LI, Skorecki KL, Itzkovitz S, Yehezkel S, Segev Y, Shachar H, Berkovitz R, Adir Y, Vulto I, Lansdorp PM & Selig S. Telomere elongation followed by telomere length reduction, in leukocytes from divers exposed to intense oxidative stress-implications for tissue and organismal aging.  Mech Ageing Dev 132 (3): 123-30, 2011. View Abstract

Kimura M, Gazitt Y, Cao X, Zhao X, Lansdorp PM & Aviv A. Synchrony of telomere length among hematopoietic cells.  Exp Hematol 38: 854-859, 2010. View Abstract

Uringa EJ, Youds JL, Lisaingo K, Lansdorp PM & Boulton SJ. RTEL1: an essential helicase for telomere maintenance and the regulation of homologous recombination.  Nucleic Acids Res 39: 1647-55, 2010. View Abstract

Chiang YJ, Calado RT, HathcockKS, Lansdorp PM, Young NS & Hodes RJ. Telomere length is inherited with resetting of the telomere set point.  Proc Natl Acad Sci USA 107: 10148-10153, 2010. View Abstract

Falconer E, Chavez E, Henderson A & Lansdorp PM. Chromosome orientation fluorescence in situ hybridization to study sister chromatid segregation in vivo.  Nat Protoc 5: 1362-1377, 2010. View Abstract

Falconer E, Chavez EA, Henderson A, Poon SSS, McKinney S, Brown L, Huntsman DG & Lansdorp PM. Identification of sister chromatids by DNA template strand sequences.  Nature 463:93-7, 2010. View Abstract

Gourronc FA, Robertson MM, Herrig AK, Lansdorp PM, Goldman FD, & Klingelhutz AJ. Proliferative defects in dyskeratosis congenita skin keratinocytes are corrected by expression of the telomerase reverse transcriptase, TERT, or by activation of endogenous telomerase through expression of papillomavirus E6/E7 or the telomerase RNA component, TERC. Exp Dermatol 19 (3): 279-88, 2010. View Abstract

Calado RT, Regal JA, KleinerDE, Schrump DS, Peterson NR, Pons V, Chanock SJ, Lansdorp PM, & Young NS. A spectrum of severe familial liver disorders associate with telomerase mutations. PLoS One 4 (11): e7926, 2009. View Abstract

Hills M, & Lansdorp PM. Short telomeres resulting from heritable mutations in the telomerase reverse transcriptase gene predispose for a variety of malignancies. Ann N Y Acad Sci 1176: 178-90, 2009. View Abstract

Breakey VR, Meyn S, Ng V, Allen C, Dokal I, Lansdorp PM, Abla O, & Dror Y. Hepatitis-associated aplastic anemia presenting as a familial bone marrow failure syndrome. J Pediatr Hematol Oncol 31 (11): 884-7, 2009. View Abstract

Lansdorp PM. Telomeres and disease. EMBO J 28 (17): 2532-40 2009. View abstract

Banath JP, Banuelos CA, Klokov D, MacPhail SM, Lansdorp PM, & Olive PL. Explanation for excessive DNA single-strand breaks and endogenous repair foci in pluripotent mouse embryonic stem cells. Exp Cell Res 315 (8): 1505-20, 2009. View Abstract

Calado RT, Regal JA, Hills M, Yewdell WT, Dalmazzo LF, Zago MA, Lansdorp PM, Hogge D, Chanock SJ, Estey EH, Falcao RP, & Young NS. Constitutional hypomorphic telomerase mutations in patients with acute myeloid leukemia. Proc Natl Acad Sci U S A 106 (4): 1187-92, 2009. View Abstract

Decker ML, Chavez E, Vulto I, & Lansdorp PM. Telomere length in Hutchinson-Gilford progeria syndrome. Mech Ageing Dev 130 (6): 377-83, 2009. View Abstract

Hills M, Lucke K, Chavez EA, Eaves CJ, & Lansdorp PM. Probing the mitotic history and developmental stage of hematopoietic cells using single telomere length analysis (STELA). Blood 113 (23): 5765-75, 2009. View Abstract

Alder JK, Chen JJ, Lancaster L, Danoff S, Su SC, Cogan JD, Vulto I, Xie M, Qi X, Tuder RM, Phillips JA, 3rd, Lansdorp PM, Loyd JE, & Armanios MY. Short telomeres are a risk factor for idiopathic pulmonary fibrosis. Proc Natl Acad Sci U S A 105 (35): 13051-6, 2008. View Abstract

Aubert G, & Lansdorp PM. Telomeres and aging. Physiol Rev 88 (2): 557-79, 2008. View Abstract

Banuelos CA, Banath JP, MacPhail SH, Zhao J, Eaves CA, O’Connor MD, Lansdorp PM, & Olive PL. Mouse but not human embryonic stem cells are deficient in rejoining of ionizing radiation-induced DNA double-strand breaks. DNA Repair (Amst) 7 (9): 1471-83, 2008. View Abstract

Berger C, Jensen MC, Lansdorp PM, Gough M, Elliott C, & Riddell SR. Adoptive transfer of effector CD8+ T cells derived from central memory cells establishes persistent T cell memory in primates. J Clin Invest 118 (1): 294-305, 2008. View Abstract

Goldman FD, Aubert G, Klingelhutz AJ, Hills M, Cooper SR, Hamilton WS, Schlueter AJ, Lambie K, Eaves CJ, & Lansdorp PM. Characterization of primitive hematopoietic cells from patients with dyskeratosis congenita. Blood 111 (9): 4523-31, 2008. View Abstract

Halaschek-Wiener J, Vulto I, Fornika D, Collins J, Connors JM, Le ND, Lansdorp PM, & Brooks-Wilson A. Reduced telomere length variation in healthy oldest old. Mech Ageing Dev 129 (11): 638-41, 2008. View Abstract

Liu Y, Bohr VA & Lansdorp P. Telomere, telomerase and aging. Mech Ageing Dev 129: 1-2, 2008. View Abstract

Berger C, Jensen MC, Lansdorp PM,Gough M, Elliott C & Riddell SR. Adoptive transfer of effector CD8+ T cells derived from central memory cells establishes persistent T cell memory in primates. J Clin Invest 118: 294-305, 2008. View Abstract

Goldman FD, Aubert G, Klingelhutz AJ, Hills M, Cooper SR, Hamilton WS, Schlueter A, Lambie K, Eaves CJ & Lansdorp PM. Characterization of primitive hematopoietic cells from patients with dyskeratosis congenita. Blood 111 (9): 4523-31, 2008. View Abstract

Lansdorp PM. Telomeres, stem cells and hematology. Blood 111: 1759-1766, 2008. View Abstract

Savage SA, Giri N, Baerlocher GM, Orr N, Lansdorp PM & Alter BP. TINF2, a component of the Shelterin telomere protection complex, is mutated in dyskeratosis congenita. Am J Hum Genet 82(2): 501-9, 2008. View Abstract

Alter BP, Baerlocher GM, Savage SA, Chanock SJ, Weksler BB, Willner JP, Peters JA, Giri N, & Lansdorp PM. Very short telomere length by flow fluorescence in situ hybridization identifies patients with dyskeratosis congenita. Blood 110 (5): 1439-47, 2007. View Abstract

Armanios MY, Chen JJ, Cogan JD, Alder JK, Ingersoll RG, Markin C, Lawson WE, Xie M, Vulto I, Phillips JA, 3rd, Lansdorp PM, Greider CW, & Loyd JE. Telomerase mutations in families with idiopathic pulmonary fibrosis. N Engl J Med 356 (13): 1317-26, 2007. View Abstract

Baerlocher GM, Rice K, Vulto I, & Lansdorp PM. Longitudinal data on telomere length in leukocytes from newborn baboons support a marked drop in stem cell turnover around 1 year of age. Aging Cell 6 (1): 121-3, 2007. View Abstract

Lansdorp PM. Immortal strands? Give me a break. Cell 129 (7): 1244-7, 2007. View Abstract

Baerlocher GM, Vulto I, de Jong G, & Lansdorp PM. Flow cytometry and FISH to measure the average length of telomeres (flow FISH). Nat Protoc 1 (5): 2365-76, 2006. View Abstract

Cheung I, Schertzer M, Rose A, & Lansdorp PM. High incidence of rapid telomere loss in telomerase-deficient Caenorhabditis elegans. Nucleic Acids Res 34 (1): 96-103, 2006. View Abstract

Lansdorp PM. Stress, social rank and leukocyte telomere length. Aging Cell 5 (6): 583-4, 2006. View Abstract

Lansdorp PM. Major cutbacks at chromosome ends. Trends Biochem Sci 30 (7): 388-95, 2005. View Abstract

Ly H, Schertzer M, Jastaniah W, Davis J, Yong SL, Ouyang Q, Blackburn EH, Parslow TG, & Lansdorp PM. Identification and functional characterization of 2 variant alleles of the telomerase RNA template gene (TERC) in a patient with dyskeratosis congenita. Blood 106 (4): 1246-52, 2005. View Abstract

Yamaguchi H, Calado RT, Ly H, Kajigaya S, Baerlocher GM, Chanock SJ, Lansdorp PM, & Young NS. Mutations in TERT, the gene for telomerase reverse transcriptase, in aplastic anemia. N Engl J Med 352 (14): 1413-24, 2005. View Abstract

Cheung I, Schertzer M, Baross A, Rose AM, Lansdorp PM, & Baird DM. Strain-specific telomere length revealed by single telomere length analysis in Caenorhabditis elegans. Nucleic Acids Res 32 (11): 3383-91, 2004. View Abstract

Ding H, Schertzer M, Wu X, Gertsenstein M, Selig S, Kammori M, Pourvali R, Poon S, Vulto I, Chavez E, Tam PP, Nagy A, & Lansdorp PM. Regulation of murine telomere length by Rtel: an essential gene encoding a helicase-like protein. Cell 117 (7): 873-86, 2004. View Abstract

Baerlocher GM, Mak J, Roth A, Rice KS, & Lansdorp PM. Telomere shortening in leukocyte subpopulations from baboons. J Leukoc Biol 73 (2): 289-96, 2003. View Abstract

Fogarty PF, Yamaguchi H, Wiestner A, Baerlocher GM, Sloand E, Zeng WS, Read EJ, Lansdorp PM, & Young NS. Late presentation of dyskeratosis congenita as apparently acquired aplastic anaemia due to mutations in telomerase RNA. Lancet 362 (9396): 1628-30, 2003. View Abstract

Roth A, Vercauteren S, Sutherland HJ, & Lansdorp PM. Telomerase is limiting the growth of acute myeloid leukemia cells. Leukemia 17 (12): 2410-7, 2003. View Abstract

Roth A, Yssel H, Pene J, Chavez EA, Schertzer M, Lansdorp PM, Spits H, & Luiten RM. Telomerase levels control the lifespan of human T lymphocytes. Blood 102 (3): 849-57, 2003. View Abstract

Tchirkov A, & Lansdorp PM. Role of oxidative stress in telomere shortening in cultured fibroblasts from normal individuals and patients with ataxia-telangiectasia. Hum Mol Genet 12 (3): 227-32, 2003. View Abstract

Van Ziffle JA, Baerlocher GM, & Lansdorp PM. Telomere length in subpopulations of human hematopoietic cells. Stem Cells 21 (6): 654-60, 2003. View Abstract

Yamaguchi H, Baerlocher GM, Lansdorp PM, Chanock SJ, Nunez O, Sloand E, & Young NS. Mutations of the human telomerase RNA gene (TERC) in aplastic anemia and myelodysplastic syndrome. Blood 102 (3): 916-8, 2003. View Abstract

Cheung I, Schertzer M, Rose A, & Lansdorp PM. Disruption of dog-1 in Caenorhabditis elegans triggers deletions upstream of guanine-rich DNA. Nat Genet 31 (4): 405-9, 2002. View Abstract

Verfaillie CM, Pera MF, & Lansdorp PM. Stem cells: hype and reality. Hematology Am Soc Hematol Educ Program: 369-91, 2002. View Abstract

Brummendorf TH, Maciejewski JP, Mak J, Young NS, & Lansdorp PM. Telomere length in leukocyte subpopulations of patients with aplastic anemia. Blood 97 (4): 895-900, 2001. View Abstract

Hande MP, Balajee AS, Tchirkov A, Wynshaw-Boris A, & Lansdorp PM. Extra-chromosomal telomeric DNA in cells from Atm(-/-) mice and patients with ataxia-telangiectasia. Hum Mol Genet 10 (5): 519-28, 2001. View Abstract

Poon SS, & Lansdorp PM. Measurements of telomere length on individual chromosomes by image cytometry. Methods Cell Biol 64: 69-96, 2001. View Abstract

Rufer N, Brummendorf TH, Chapuis B, Helg C, Lansdorp PM, & Roosnek E. Accelerated telomere shortening in hematological lineages is limited to the first year following stem cell transplantation. Blood 97 (2): 575-7, 2001. View Abstract

Brummendorf TH, Holyoake TL, Rufer N, Barnett MJ, Schulzer M, Eaves CJ, Eaves AC, & Lansdorp PM. Prognostic implications of differences in telomere length between normal and malignant cells from patients with chronic myeloid leukemia measured by flow cytometry. Blood 95 (6): 1883-90, 2000. View Abstract

Lansdorp PM. Repair of telomeric DNA prior to replicative senescence. Mech Ageing Dev 118 (1-2): 23-34, 2000. View Abstract

Lanza RP, Cibelli JB, Blackwell C, Cristofalo VJ, Francis MK, Baerlocher GM, Mak J, Schertzer M, Chavez EA, Sawyer N, Lansdorp PM, & West MD. Extension of cell life-span and telomere length in animals cloned from senescent somatic cells. Science 288 (5466): 665-9, 2000. View Abstract

Liu Y, Snow BE, Hande MP, Baerlocher G, Kickhoefer VA, Yeung D, Wakeham A, Itie A, Siderovski DP, Lansdorp PM, Robinson MO, & Harrington L. Telomerase-associated protein TEP1 is not essential for telomerase activity or telomere length maintenance in vivo. Mol Cell Biol 20 (21): 8178-84, 2000. View Abstract

Martens UM, Chavez EA, Poon SS, Schmoor C, & Lansdorp PM. Accumulation of short telomeres in human fibroblasts prior to replicative senescence. Exp Cell Res 256 (1): 291-9, 2000. View Abstract

d’Adda di Fagagna F, Hande MP, Tong WM, Lansdorp PM, Wang ZQ, & Jackson SP. Functions of poly(ADP-ribose) polymerase in controlling telomere length and chromosomal stability. Nat Genet 23 (1): 76-80, 1999. View Abstract

Hande MP, Samper E, Lansdorp P, & Blasco MA. Telomere length dynamics and chromosomal instability in cells derived from telomerase null mice. J Cell Biol 144 (4): 589-601, 1999. View Abstract

Poon SS, Martens UM, Ward RK, & Lansdorp PM. Telomere length measurements using digital fluorescence microscopy. Cytometry 36 (4): 267-78, 1999. View Abstract

Rufer N, Brummendorf TH, Kolvraa S, Bischoff C, Christensen K, Wadsworth L, Schulzer M, & Lansdorp PM. Telomere fluorescence measurements in granulocytes and T lymphocyte subsets point to a high turnover of hematopoietic stem cells and memory T cells in early childhood. J Exp Med 190 (2): 157-67, 1999. View Abstract

Surralles J, Hande MP, Marcos R, & Lansdorp PM. Accelerated telomere shortening in the human inactive X chromosome. Am J Hum Genet 65 (6): 1617-22, 1999. View Abstract

Wan TS, Martens UM, Poon SS, Tsao SW, Chan LC, & Lansdorp PM. Absence or low number of telomere repeats at junctions of dicentric chromosomes. Genes Chromosomes Cancer 24 (1): 83-6, 1999. View Abstract

Brummendorf TH, Dragowska W, Zijlmans J, Thornbury G, & Lansdorp PM. Asymmetric cell divisions sustain long-term hematopoiesis from single-sorted human fetal liver cells. J Exp Med 188 (6): 1117-24, 1998. View Abstract

Martens UM, Zijlmans JM, Poon SS, Dragowska W, Yui J, Chavez EA, Ward RK, & Lansdorp PM. Short telomeres on human chromosome 17p. Nat Genet 18 (1): 76-80, 1998. View Abstract

Rufer N, Dragowska W, Thornbury G, Roosnek E, & Lansdorp PM. Telomere length dynamics in human lymphocyte subpopulations measured by flow cytometry. Nat Biotechnol 16 (8): 743-7, 1998. View Abstract

Yui J, Chiu CP, & Lansdorp PM. Telomerase activity in candidate stem cells from fetal liver and adult bone marrow. Blood 91 (9): 3255-62, 1998. View Abstract

Blasco MA, Lee HW, Hande MP, Samper E, Lansdorp PM, DePinho RA, & Greider CW. Telomere shortening and tumor formation by mouse cells lacking telomerase RNA. Cell 91 (1): 25-34, 1997. View Abstract

Lansdorp PM. Self-renewal of stem cells. Biol Blood Marrow Transplant 3 (4): 171-8, 1997. View Abstract

Zijlmans JM, Martens UM, Poon SS, RaapAK, Tanke HJ, Ward RK, & Lansdorp PM. Telomeres in the mouse have large inter-chromosomal variations in the number of T2AG3 repeats. Proc Natl Acad Sci U S A 94 (14): 7423-8, 1997. View Abstract

Chiu CP, Dragowska W, Kim NW, Vaziri H, Yui J, Thomas TE, Harley CB, & Lansdorp PM. Differential expression of telomerase activity in hematopoietic progenitors from adult human bone marrow. Stem Cells 14 (2): 239-48, 1996. View Abstract

Lansdorp PM, Verwoerd NP, van de Rijke FM, Dragowska V, Little MT, Dirks RW, Raap AK, & Tanke HJ. Heterogeneity in telomere length of human chromosomes. Hum Mol Genet 5 (5): 685-91, 1996. View Abstract

Rebel VI, Miller CL, Eaves CJ, & Lansdorp PM. The repopulation potential of fetal liver hematopoietic stem cells in mice exceeds that of their liver adult bone marrow counterparts. Blood 87 (8): 3500-7, 1996. View Abstract

Sauvageau G, Thorsteinsdottir U, Eaves CJ, Lawrence HJ, Largman C, Lansdorp PM, & Humphries RK. Overexpression of HOXB4 in hematopoietic cells causes the selective expansion of more primitive populations in vitro and in vivo. Genes Dev 9 (14): 1753-65, 1995. View Abstract

Vaziri H, Dragowska W, Allsopp RC, Thomas TE, Harley CB, & Lansdorp PM. Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age. Proc Natl Acad Sci U S A 91 (21): 9857-60, 1994. View Abstract

Lansdorp PM, Dragowska W, & Mayani H. Ontogeny-related changes in proliferative potential of human hematopoietic cells. J Exp Med 178 (3): 787-91, 1993. View Abstract

Lansdorp PM, & Dragowska W. Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow. J Exp Med 175 (6): 1501-9, 1992. View Abstract

Lansdorp PM, Sutherland HJ, & Eaves CJ. Selective expression of CD45 isoforms on functional subpopulations of CD34+ hemopoietic cells from human bone marrow. J Exp Med 172 (1): 363-6, 1990. View Abstract

Sutherland HJ, Eaves CJ, Eaves AC, Dragowska W, & Lansdorp PM. Characterization and partial purification of human marrow cells capable of initiating long-term hematopoiesis in vitro. Blood 74 (5): 1563-70, 1989. View Abstract

van den Engh G, Trask B, Lansdorp P, & Gray J. Improved resolution of flow cytometric measurements of Hoechst- and chromomycin-A3-stained human chromosomes after addition of citrate and sulfite. Cytometry 9 (3): 266-70, 1988. View Abstract

Lansdorp PM, Aalberse RC, Bos R, Schutter WG, & Van Bruggen EF. Cyclic tetramolecular complexes of monoclonal antibodies: a new type of cross-linking reagent. Eur J Immunol 16 (6): 679-83, 1986. View Abstract

 

Vancouver, BC --  22 October 2013 -- Amy Yu - Terry Fox Laboratory (Photo: BC Cancer Agency)

Amy Yu

PMP
Research Projects Manager
M Yuen

Michael Yuen

Graduate Student