People

Principal Investigators

Back

Dr Peter Lansdorp — MD, PhD, FRS(C)

Distinguished Scientist

P Lansdorp1

Admin Contact

Shannon Valdez

Research Projects Coordinator
  604-675-8128
Overview
Research Interest
Publications
Lab Members

Education:
MD, Erasmus University Rotterdam, 1976
PhD, Experimental Hematology, University of Amsterdam, 1985

  • Asymmetric cell divisions and stem cell biology
  • Genetic instability, aging and cancer
  • Replication and repair of G-rich DNA and telomere repeats
  • Fluorescence in situ hybridization and flow cytometry

Asymmetric cell divisions are required for the development of all multi-cellular organisms. Many mechanisms are known to help secure differences between daughter cells following a parental cell division. We are interested in exploring the possibility that differences in parental cells before they divide contribute to differences between daughter cells as well. For example, it seems possible that chromatin differences between sister chromatids co-regulate the expression of specific genes as predicted by the “silent sister hypothesis” (1). In order to test this hypothesis it is necessary to reliably distinguish between sister chromatids. We recently described a novel approaches to identify and study sister chromatids (2,3).  Current studies focus on testing the “silent sister hypothesis” using cells that divide asymmetrically and cells in which asymmetry is not obvious such as self-renewing normal and malignant (stem) cells.

The second area of interest is the role of genome stability in aging. Accurate replication of DNA and faithful segregation of chromosomes are essential for all forms of life. In multi-cellular organisms, the control of these processes differs markedly between various cell types and between organisms. For example, the number of cell divisions is tightly controlled in adult blood-forming stem cells, whereas embryonic stem cells are essentially immortal. Furthermore, most adult stem cells are typically quiescent, whereas embryonic stem cells turnover rapidly. The number of cell divisions that adult stem cells can undergo seems much more tightly controlled in humans than in mice, perhaps because tumor growth poses a greater risk to reproductive fitness in humans (with a longer lifespan and a larger body mass) than in mice (4). We previously engineered murine ES cells (5) with specific defects in the replication of chromosome ends (telomeres). Surprisingly, such cells appear to grow normally until differentiation is induced. In order to study the molecular mechanisms that underpin these observations, we are using these and other ES cells expressing fluorescent reporter constructs before and after various genetic manipulations (6). The goal of these studies is to characterize pathways involved in cell cycle checkpoints, telomere maintenance and DNA damage responses in ES cells before and after induction of differentiation. The single cell sequencing approaches developed in our laboratory have great potential to address many questions in biology including questions about genome stability, heritable as well as sporadic genetic rearrangements, reference genome assembly and haplotyping (7,8)

In all vertebrates telomeres are characterized by TTAGGG repeats and associated proteins. A minimum number of telomere repeats is needed at each end in order to distinguish a normal end form a double strand break and protect chromosome ends from fusion and degradation. Critically short or truncated telomeres trigger a DNA damage response that can cause cell cycle arrest or cell death. Telomeric DNA is lost with each round of cell division via several distinct pathways (9). Lost telomere repeats are normally replenished by the enzyme telomerase, a reverse transcriptase which can add telomere repeats to the 3’ end of chromosomes using an RNA template. Telomerase levels appear to be limiting in normal human blood forming stem cells (10). We have developed novel tools to measure the telomere length in individual cells and individual chromosomes (11,12) and these quantitative fluorescence in situ hybridization techniques are used to address questions about the role of telomeres in normal aging, tumor progression and specific genetic, hematological and immune disorders. Based on our studies in C.elegans (13) we are particularly interested in guanine-rich DNA capable of forming higher order structures known guanine quadruplex (G4) DNA. We have developed a panel of monoclonal antibodies against different G4 DNA structures (14) to study on the role of G4 DNA in the regulation of gene expression and telomere biology.

1.         Lansdorp, P.M. (2007) Immortal strands? Give me a break. Cell, 129, 1244-1247.

2.         Falconer, E., Chavez, E.A., Henderson, A., Poon, S.S., McKinney, S., Brown, L., Huntsman, D.G. and Lansdorp, P.M. (2010) Identification of sister chromatids by DNA template strand sequences. Nature, 463, 93-97.

3.         Falconer, E., Hills, M., Naumann, U., Poon, S.S., Chavez, E.A., Sanders, A.D., Zhao, Y., Hirst, M. and Lansdorp, P.M. (2012) DNA template strand sequencing of single-cells maps genomic rearrangements at high resolution. Nat Methods, 9, 1107-1112.

4.         Lansdorp, P.M. (2009) Telomeres and disease. The EMBO journal, 28, 2532-2540.

5.         Ding, H., Schertzer, M., Wu, X., Gertsenstein, M., Selig, S., Kammori, M., Pourvali, R., Poon, S., Vulto, I., Chavez, E. et al. (2004) Regulation of murine telomere length by Rtel: an essential gene encoding a helicase-like protein. Cell, 117, 873-886.

6.         Lisaingo, K., Uringa, E.J. and Lansdorp, P.M. (2014) Resolution of telomere associations by TRF1 cleavage in mouse embryonic stem cells. Mol Biol Cell.

7.         Falconer, E. and Lansdorp, P.M. (2013) Strand-seq: A unifying tool for studies of chromosome segregation. Seminars in cell & developmental biology.

8.         Hills, M., O'Neill, K., Falconer, E., Brinkman, R. and Lansdorp, P.M. (2013) BAIT: Organizing genomes and mapping rearrangements in single cells. Genome medicine, 5, 82.

9.         Lansdorp, P.M. (2005) Major cutbacks at chromosome ends. Trends Biochem Sci, 30, 388-395.

10.       Aubert, G., Baerlocher, G.M., Vulto, I., Poon, S.S. and Lansdorp, P.M. (2012) Collapse of telomere homeostasis in hematopoietic cells caused by heterozygous mutations in telomerase genes. PLoS Genet, 8, e1002696.

11.       Lansdorp, P.M., Verwoerd, N.P., van de Rijke, F.M., Dragowska, V., Little, M.T., Dirks, R.W., Raap, A.K. and Tanke, H.J. (1996) Heterogeneity in telomere length of human chromosomes. Human molecular genetics, 5, 685-691.

12.       Baerlocher, G.M., Vulto, I., de Jong, G. and Lansdorp, P.M. (2006) Flow cytometry and FISH to measure the average length of telomeres (flow FISH). Nat Protoc, 1, 2365-2376.

13.       Cheung, I., Schertzer, M., Rose, A. and Lansdorp, P.M. (2002) Disruption of dog-1 in Caenorhabditis elegans triggers deletions upstream of guanine-rich DNA. Nat Genet, 31, 405-409.

14.       Henderson, A., Wu, Y., Huang, Y.C., Chavez, E.A., Platt, J., Johnson, F.B., Brosh, R.M., Jr., Sen, D. and Lansdorp, P.M. (2014) Detection of G-quadruplex DNA in mammalian cells. Nucleic acids research, 42, 860-869.

Gardner M, Bann D, Wiley L, Cooper R, Hardy R, Nitsch D, Martin-Ruiz C, Shiels P, Sayer AA, Barbieri M, Bekaert S, Bischoff C, Brooks-Wilson A, Chen W, Cooper C, Christensen K, De Meyer T, Deary I, Der G, Diez Roux A, Fitzpatrick A, Hajat A, Halaschek-Wiener J, Harris S, Hunt SC, Jaggar C, Jeon HS, Kaplan R, Kimura M, Lansdorp P, Li C, Maeda T, Mangino M, Nawrot TS, Nilsson P, Nordfjall K, Paolisso G, Ren F, Riabowol K, Robertson T, Roos G, Staessen JA, Spector T, Tang N, Unryn B, van der Harst P, Woo J, Xing C, Yadegarfar ME, Park JY, Young N, Kuh D, von Zglinicki T, Ben-Shlomo Y & Halcyon study team. Gender and telomere length: Systematic review and meta-analysis.  Exp Gerontol 51: 15-27, 2014. View Abstract

Henderson A, Wu Y, Huang YC, Chavez EA, Platt J, Johnson FB, Brosh RM, Jr., Sen D & Lansdorp PM. Detection of G-quadruplex DNA in mammalian cells.  Nucleic Acids Res 42: 860-869, 2014. View Abstract

Falconer E & Lansdorp PM. Strand-seq: A unifying tool for studies of chromosome segregation. Semin Cell Dev Biol 24: 643-652, 2013. View Abstract

Hills M, O'Neil K, Falconer E, Brinkman R & Lansdorp PM. BAIT:Organizing genomes and mapping rearrangements in single cells.  Genome Med 5: 82 2013. View Abstract

Kannan N, Huda N, Tu L, Droumeva R, Aubert G, Chavez E, Brinkman RR, Lansdorp P, Emerman J, Abe S, Eaves C & Gilley D. The luminal progenitor compartment of the normal human mammary gland constitutes a unique site of telomere dysfunction.  Stem Cell Reports 1: 28-37, 2013. View Abstract

Falconer E, Hills M, Naumann U, Poon SS, Chavez EA, Sanders AD, Hirst M & Lansdorp PM. DNA template strand sequencing of single-cells maps genomic rearrangements at high resolution.  Nature Methods 9: 1107-1112, 2012. View Abstract

van Wietmarschen N, Moradian A, Morin GB, Lansdorp PM & Uringa EJ. The mammalian proteins MMS19, MIP18, and ANT2 are involved in cytoplasmic iron-sulfur cluster protein assembly.  J Biol Chem 287: 43351-43358, 2012. View Abstract

Frumento G, Zheng Y, Aubert G, Raeiszadeh M, Lansdorp PM, Moss P, Lee SP & Chen FE. Cord blood T cells retain early differentiation phenotype suitable for immunotherapy after TCR gene transfer to confer EBV specificity.  Am J Transplant 13: 45-55, 2012. View Abstract

Lansdorp PM, Falconer E, Tao J, Brind'Amour J & Naumann R. Epigenetic differences between sister chromatids.  Ann N Y Acad Sci 1266: 1-6, 2012. View Abstract

Uringa E-J, Lisaingo K, Pickett HA, Brind'Amour J, Rohde J-H, Zelensky A, Essers J & Lansdorp PM. RTEL1 contributes to DNA replication, repair and telomere maintenance. Mol Biol Cell 23: 2782-92, 2012. View Abstract

Aubert G, Baerlocher GM, Vulto I, Poon SS & Lansdorp PM. Collapse of telomere homeostasis in hematopoietic stem cells caused by heterozygous mutations in telomerase genes. PLoS Genet 8: e1002696, 2012. View Paper

Alter BP, Rosenberg PS, Giri N, Lansdorp PM & Savage SA. Telomere length is associated with disease severity and declines with age in dyskeratosis congenita. Haematologica 97: 353-539, 2012. View Abstract

Sekulovic S, Gylfadottir V, Vulto I, Gasparetto M, Even Y, Brookes C, Smith C, Eaves CJ, Lansdorp PM, Rossi FM & Humphries RK. Prolonged self-renewal activity unmasks telomerase control of telomere homeostasis and function of mouse hematopoietic stem cells. Blood 118: 1766-73, 2011. View Abstract

Aubert G, Hills M & Lansdorp PM. Telomere length measurement-Caveats and a critical assessment of the available technologies and tools. Mutat Res 730: 59-67, 2012. View Abstract

Brind'Amour J & Lansdorp PM. Analysis of repetitive DNA in chromosomes by flow cytometry.  Nat Methods 8: 484-486, 2011. View Abstract

Alder JK, Cogan JD, Brown AF, Anderson CJ, Lawson WE, Lansdorp PM, Philips JA, Loyd JE, Chen JJ & Armanios MY. Ancestral mutation in telomerase causes defects in repeat addition processivity and manifests as familial pulmonary fibrosis.  PLoS Genet 7: e1001352 2011. View Abstract

Shlush LI, Skorecki KL, Itzkovitz S, Yehezkel S, Segev Y, Shachar H, Berkovitz R, Adir Y, Vulto I, Lansdorp PM & Selig S. Telomere elongation followed by telomere length reduction, in leukocytes from divers exposed to intense oxidative stress-implications for tissue and organismal aging.  Mech Ageing Dev 132 (3): 123-30, 2011. View Abstract

Kimura M, Gazitt Y, Cao X, Zhao X, Lansdorp PM & Aviv A. Synchrony of telomere length among hematopoietic cells.  Exp Hematol 38: 854-859, 2010. View Abstract

Uringa EJ, Youds JL, Lisaingo K, Lansdorp PM & Boulton SJ. RTEL1: an essential helicase for telomere maintenance and the regulation of homologous recombination.  Nucleic Acids Res 39: 1647-55, 2010. View Abstract

Chiang YJ, Calado RT, HathcockKS, Lansdorp PM, Young NS & Hodes RJ. Telomere length is inherited with resetting of the telomere set point.  Proc Natl Acad Sci USA 107: 10148-10153, 2010. View Abstract

Falconer E, Chavez E, Henderson A & Lansdorp PM. Chromosome orientation fluorescence in situ hybridization to study sister chromatid segregation in vivo.  Nat Protoc 5: 1362-1377, 2010. View Abstract

Falconer E, Chavez EA, Henderson A, Poon SSS, McKinney S, Brown L, Huntsman DG & Lansdorp PM. Identification of sister chromatids by DNA template strand sequences.  Nature 463:93-7, 2010. View Abstract

Gourronc FA, Robertson MM, Herrig AK, Lansdorp PM, Goldman FD, & Klingelhutz AJ. Proliferative defects in dyskeratosis congenita skin keratinocytes are corrected by expression of the telomerase reverse transcriptase, TERT, or by activation of endogenous telomerase through expression of papillomavirus E6/E7 or the telomerase RNA component, TERC. Exp Dermatol 19 (3): 279-88, 2010. View Abstract

Calado RT, Regal JA, KleinerDE, Schrump DS, Peterson NR, Pons V, Chanock SJ, Lansdorp PM, & Young NS. A spectrum of severe familial liver disorders associate with telomerase mutations. PLoS One 4 (11): e7926, 2009. View Abstract

Hills M, & Lansdorp PM. Short telomeres resulting from heritable mutations in the telomerase reverse transcriptase gene predispose for a variety of malignancies. Ann N Y Acad Sci 1176: 178-90, 2009. View Abstract

Breakey VR, Meyn S, Ng V, Allen C, Dokal I, Lansdorp PM, Abla O, & Dror Y. Hepatitis-associated aplastic anemia presenting as a familial bone marrow failure syndrome. J Pediatr Hematol Oncol 31 (11): 884-7, 2009. View Abstract

Lansdorp PM. Telomeres and disease. EMBO J 28 (17): 2532-40 2009. View abstract

Banath JP, Banuelos CA, Klokov D, MacPhail SM, Lansdorp PM, & Olive PL. Explanation for excessive DNA single-strand breaks and endogenous repair foci in pluripotent mouse embryonic stem cells. Exp Cell Res 315 (8): 1505-20, 2009. View Abstract

Calado RT, Regal JA, Hills M, Yewdell WT, Dalmazzo LF, Zago MA, Lansdorp PM, Hogge D, Chanock SJ, Estey EH, Falcao RP, & Young NS. Constitutional hypomorphic telomerase mutations in patients with acute myeloid leukemia. Proc Natl Acad Sci U S A 106 (4): 1187-92, 2009. View Abstract

Decker ML, Chavez E, Vulto I, & Lansdorp PM. Telomere length in Hutchinson-Gilford progeria syndrome. Mech Ageing Dev 130 (6): 377-83, 2009. View Abstract

Hills M, Lucke K, Chavez EA, Eaves CJ, & Lansdorp PM. Probing the mitotic history and developmental stage of hematopoietic cells using single telomere length analysis (STELA). Blood 113 (23): 5765-75, 2009. View Abstract

Alder JK, Chen JJ, Lancaster L, Danoff S, Su SC, Cogan JD, Vulto I, Xie M, Qi X, Tuder RM, Phillips JA, 3rd, Lansdorp PM, Loyd JE, & Armanios MY. Short telomeres are a risk factor for idiopathic pulmonary fibrosis. Proc Natl Acad Sci U S A 105 (35): 13051-6, 2008. View Abstract

Aubert G, & Lansdorp PM. Telomeres and aging. Physiol Rev 88 (2): 557-79, 2008. View Abstract

Banuelos CA, Banath JP, MacPhail SH, Zhao J, Eaves CA, O’Connor MD, Lansdorp PM, & Olive PL. Mouse but not human embryonic stem cells are deficient in rejoining of ionizing radiation-induced DNA double-strand breaks. DNA Repair (Amst) 7 (9): 1471-83, 2008. View Abstract

Berger C, Jensen MC, Lansdorp PM, Gough M, Elliott C, & Riddell SR. Adoptive transfer of effector CD8+ T cells derived from central memory cells establishes persistent T cell memory in primates. J Clin Invest 118 (1): 294-305, 2008. View Abstract

Goldman FD, Aubert G, Klingelhutz AJ, Hills M, Cooper SR, Hamilton WS, Schlueter AJ, Lambie K, Eaves CJ, & Lansdorp PM. Characterization of primitive hematopoietic cells from patients with dyskeratosis congenita. Blood 111 (9): 4523-31, 2008. View Abstract

Halaschek-Wiener J, Vulto I, Fornika D, Collins J, Connors JM, Le ND, Lansdorp PM, & Brooks-Wilson A. Reduced telomere length variation in healthy oldest old. Mech Ageing Dev 129 (11): 638-41, 2008. View Abstract

Liu Y, Bohr VA & Lansdorp P. Telomere, telomerase and aging. Mech Ageing Dev 129: 1-2, 2008. View Abstract

Berger C, Jensen MC, Lansdorp PM,Gough M, Elliott C & Riddell SR. Adoptive transfer of effector CD8+ T cells derived from central memory cells establishes persistent T cell memory in primates. J Clin Invest 118: 294-305, 2008. View Abstract

Goldman FD, Aubert G, Klingelhutz AJ, Hills M, Cooper SR, Hamilton WS, Schlueter A, Lambie K, Eaves CJ & Lansdorp PM. Characterization of primitive hematopoietic cells from patients with dyskeratosis congenita. Blood 111 (9): 4523-31, 2008. View Abstract

Lansdorp PM. Telomeres, stem cells and hematology. Blood 111: 1759-1766, 2008. View Abstract

Savage SA, Giri N, Baerlocher GM, Orr N, Lansdorp PM & Alter BP. TINF2, a component of the Shelterin telomere protection complex, is mutated in dyskeratosis congenita. Am J Hum Genet 82(2): 501-9, 2008. View Abstract

Alter BP, Baerlocher GM, Savage SA, Chanock SJ, Weksler BB, Willner JP, Peters JA, Giri N, & Lansdorp PM. Very short telomere length by flow fluorescence in situ hybridization identifies patients with dyskeratosis congenita. Blood 110 (5): 1439-47, 2007. View Abstract

Armanios MY, Chen JJ, Cogan JD, Alder JK, Ingersoll RG, Markin C, Lawson WE, Xie M, Vulto I, Phillips JA, 3rd, Lansdorp PM, Greider CW, & Loyd JE. Telomerase mutations in families with idiopathic pulmonary fibrosis. N Engl J Med 356 (13): 1317-26, 2007. View Abstract

Baerlocher GM, Rice K, Vulto I, & Lansdorp PM. Longitudinal data on telomere length in leukocytes from newborn baboons support a marked drop in stem cell turnover around 1 year of age. Aging Cell 6 (1): 121-3, 2007. View Abstract

Lansdorp PM. Immortal strands? Give me a break. Cell 129 (7): 1244-7, 2007. View Abstract

Baerlocher GM, Vulto I, de Jong G, & Lansdorp PM. Flow cytometry and FISH to measure the average length of telomeres (flow FISH). Nat Protoc 1 (5): 2365-76, 2006. View Abstract

Cheung I, Schertzer M, Rose A, & Lansdorp PM. High incidence of rapid telomere loss in telomerase-deficient Caenorhabditis elegans. Nucleic Acids Res 34 (1): 96-103, 2006. View Abstract

Lansdorp PM. Stress, social rank and leukocyte telomere length. Aging Cell 5 (6): 583-4, 2006. View Abstract

Lansdorp PM. Major cutbacks at chromosome ends. Trends Biochem Sci 30 (7): 388-95, 2005. View Abstract

Ly H, Schertzer M, Jastaniah W, Davis J, Yong SL, Ouyang Q, Blackburn EH, Parslow TG, & Lansdorp PM. Identification and functional characterization of 2 variant alleles of the telomerase RNA template gene (TERC) in a patient with dyskeratosis congenita. Blood 106 (4): 1246-52, 2005. View Abstract

Yamaguchi H, Calado RT, Ly H, Kajigaya S, Baerlocher GM, Chanock SJ, Lansdorp PM, & Young NS. Mutations in TERT, the gene for telomerase reverse transcriptase, in aplastic anemia. N Engl J Med 352 (14): 1413-24, 2005. View Abstract

Cheung I, Schertzer M, Baross A, Rose AM, Lansdorp PM, & Baird DM. Strain-specific telomere length revealed by single telomere length analysis in Caenorhabditis elegans. Nucleic Acids Res 32 (11): 3383-91, 2004. View Abstract

Ding H, Schertzer M, Wu X, Gertsenstein M, Selig S, Kammori M, Pourvali R, Poon S, Vulto I, Chavez E, Tam PP, Nagy A, & Lansdorp PM. Regulation of murine telomere length by Rtel: an essential gene encoding a helicase-like protein. Cell 117 (7): 873-86, 2004. View Abstract

Baerlocher GM, Mak J, Roth A, Rice KS, & Lansdorp PM. Telomere shortening in leukocyte subpopulations from baboons. J Leukoc Biol 73 (2): 289-96, 2003. View Abstract

Fogarty PF, Yamaguchi H, Wiestner A, Baerlocher GM, Sloand E, Zeng WS, Read EJ, Lansdorp PM, & Young NS. Late presentation of dyskeratosis congenita as apparently acquired aplastic anaemia due to mutations in telomerase RNA. Lancet 362 (9396): 1628-30, 2003. View Abstract

Roth A, Vercauteren S, Sutherland HJ, & Lansdorp PM. Telomerase is limiting the growth of acute myeloid leukemia cells. Leukemia 17 (12): 2410-7, 2003. View Abstract

Roth A, Yssel H, Pene J, Chavez EA, Schertzer M, Lansdorp PM, Spits H, & Luiten RM. Telomerase levels control the lifespan of human T lymphocytes. Blood 102 (3): 849-57, 2003. View Abstract

Tchirkov A, & Lansdorp PM. Role of oxidative stress in telomere shortening in cultured fibroblasts from normal individuals and patients with ataxia-telangiectasia. Hum Mol Genet 12 (3): 227-32, 2003. View Abstract

Van Ziffle JA, Baerlocher GM, & Lansdorp PM. Telomere length in subpopulations of human hematopoietic cells. Stem Cells 21 (6): 654-60, 2003. View Abstract

Yamaguchi H, Baerlocher GM, Lansdorp PM, Chanock SJ, Nunez O, Sloand E, & Young NS. Mutations of the human telomerase RNA gene (TERC) in aplastic anemia and myelodysplastic syndrome. Blood 102 (3): 916-8, 2003. View Abstract

Cheung I, Schertzer M, Rose A, & Lansdorp PM. Disruption of dog-1 in Caenorhabditis elegans triggers deletions upstream of guanine-rich DNA. Nat Genet 31 (4): 405-9, 2002. View Abstract

Verfaillie CM, Pera MF, & Lansdorp PM. Stem cells: hype and reality. Hematology Am Soc Hematol Educ Program: 369-91, 2002. View Abstract

Brummendorf TH, Maciejewski JP, Mak J, Young NS, & Lansdorp PM. Telomere length in leukocyte subpopulations of patients with aplastic anemia. Blood 97 (4): 895-900, 2001. View Abstract

Hande MP, Balajee AS, Tchirkov A, Wynshaw-Boris A, & Lansdorp PM. Extra-chromosomal telomeric DNA in cells from Atm(-/-) mice and patients with ataxia-telangiectasia. Hum Mol Genet 10 (5): 519-28, 2001. View Abstract

Poon SS, & Lansdorp PM. Measurements of telomere length on individual chromosomes by image cytometry. Methods Cell Biol 64: 69-96, 2001. View Abstract

Rufer N, Brummendorf TH, Chapuis B, Helg C, Lansdorp PM, & Roosnek E. Accelerated telomere shortening in hematological lineages is limited to the first year following stem cell transplantation. Blood 97 (2): 575-7, 2001. View Abstract

Brummendorf TH, Holyoake TL, Rufer N, Barnett MJ, Schulzer M, Eaves CJ, Eaves AC, & Lansdorp PM. Prognostic implications of differences in telomere length between normal and malignant cells from patients with chronic myeloid leukemia measured by flow cytometry. Blood 95 (6): 1883-90, 2000. View Abstract

Lansdorp PM. Repair of telomeric DNA prior to replicative senescence. Mech Ageing Dev 118 (1-2): 23-34, 2000. View Abstract

Lanza RP, Cibelli JB, Blackwell C, Cristofalo VJ, Francis MK, Baerlocher GM, Mak J, Schertzer M, Chavez EA, Sawyer N, Lansdorp PM, & West MD. Extension of cell life-span and telomere length in animals cloned from senescent somatic cells. Science 288 (5466): 665-9, 2000. View Abstract

Liu Y, Snow BE, Hande MP, Baerlocher G, Kickhoefer VA, Yeung D, Wakeham A, Itie A, Siderovski DP, Lansdorp PM, Robinson MO, & Harrington L. Telomerase-associated protein TEP1 is not essential for telomerase activity or telomere length maintenance in vivo. Mol Cell Biol 20 (21): 8178-84, 2000. View Abstract

Martens UM, Chavez EA, Poon SS, Schmoor C, & Lansdorp PM. Accumulation of short telomeres in human fibroblasts prior to replicative senescence. Exp Cell Res 256 (1): 291-9, 2000. View Abstract

d’Adda di Fagagna F, Hande MP, Tong WM, Lansdorp PM, Wang ZQ, & Jackson SP. Functions of poly(ADP-ribose) polymerase in controlling telomere length and chromosomal stability. Nat Genet 23 (1): 76-80, 1999. View Abstract

Hande MP, Samper E, Lansdorp P, & Blasco MA. Telomere length dynamics and chromosomal instability in cells derived from telomerase null mice. J Cell Biol 144 (4): 589-601, 1999. View Abstract

Poon SS, Martens UM, Ward RK, & Lansdorp PM. Telomere length measurements using digital fluorescence microscopy. Cytometry 36 (4): 267-78, 1999. View Abstract

Rufer N, Brummendorf TH, Kolvraa S, Bischoff C, Christensen K, Wadsworth L, Schulzer M, & Lansdorp PM. Telomere fluorescence measurements in granulocytes and T lymphocyte subsets point to a high turnover of hematopoietic stem cells and memory T cells in early childhood. J Exp Med 190 (2): 157-67, 1999. View Abstract

Surralles J, Hande MP, Marcos R, & Lansdorp PM. Accelerated telomere shortening in the human inactive X chromosome. Am J Hum Genet 65 (6): 1617-22, 1999. View Abstract

Wan TS, Martens UM, Poon SS, Tsao SW, Chan LC, & Lansdorp PM. Absence or low number of telomere repeats at junctions of dicentric chromosomes. Genes Chromosomes Cancer 24 (1): 83-6, 1999. View Abstract

Brummendorf TH, Dragowska W, Zijlmans J, Thornbury G, & Lansdorp PM. Asymmetric cell divisions sustain long-term hematopoiesis from single-sorted human fetal liver cells. J Exp Med 188 (6): 1117-24, 1998. View Abstract

Martens UM, Zijlmans JM, Poon SS, Dragowska W, Yui J, Chavez EA, Ward RK, & Lansdorp PM. Short telomeres on human chromosome 17p. Nat Genet 18 (1): 76-80, 1998. View Abstract

Rufer N, Dragowska W, Thornbury G, Roosnek E, & Lansdorp PM. Telomere length dynamics in human lymphocyte subpopulations measured by flow cytometry. Nat Biotechnol 16 (8): 743-7, 1998. View Abstract

Yui J, Chiu CP, & Lansdorp PM. Telomerase activity in candidate stem cells from fetal liver and adult bone marrow. Blood 91 (9): 3255-62, 1998. View Abstract

Blasco MA, Lee HW, Hande MP, Samper E, Lansdorp PM, DePinho RA, & Greider CW. Telomere shortening and tumor formation by mouse cells lacking telomerase RNA. Cell 91 (1): 25-34, 1997. View Abstract

Lansdorp PM. Self-renewal of stem cells. Biol Blood Marrow Transplant 3 (4): 171-8, 1997. View Abstract

Zijlmans JM, Martens UM, Poon SS, RaapAK, Tanke HJ, Ward RK, & Lansdorp PM. Telomeres in the mouse have large inter-chromosomal variations in the number of T2AG3 repeats. Proc Natl Acad Sci U S A 94 (14): 7423-8, 1997. View Abstract

Chiu CP, Dragowska W, Kim NW, Vaziri H, Yui J, Thomas TE, Harley CB, & Lansdorp PM. Differential expression of telomerase activity in hematopoietic progenitors from adult human bone marrow. Stem Cells 14 (2): 239-48, 1996. View Abstract

Lansdorp PM, Verwoerd NP, van de Rijke FM, Dragowska V, Little MT, Dirks RW, Raap AK, & Tanke HJ. Heterogeneity in telomere length of human chromosomes. Hum Mol Genet 5 (5): 685-91, 1996. View Abstract

Rebel VI, Miller CL, Eaves CJ, & Lansdorp PM. The repopulation potential of fetal liver hematopoietic stem cells in mice exceeds that of their liver adult bone marrow counterparts. Blood 87 (8): 3500-7, 1996. View Abstract

Sauvageau G, Thorsteinsdottir U, Eaves CJ, Lawrence HJ, Largman C, Lansdorp PM, & Humphries RK. Overexpression of HOXB4 in hematopoietic cells causes the selective expansion of more primitive populations in vitro and in vivo. Genes Dev 9 (14): 1753-65, 1995. View Abstract

Vaziri H, Dragowska W, Allsopp RC, Thomas TE, Harley CB, & Lansdorp PM. Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age. Proc Natl Acad Sci U S A 91 (21): 9857-60, 1994. View Abstract

Lansdorp PM, Dragowska W, & Mayani H. Ontogeny-related changes in proliferative potential of human hematopoietic cells. J Exp Med 178 (3): 787-91, 1993. View Abstract

Lansdorp PM, & Dragowska W. Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow. J Exp Med 175 (6): 1501-9, 1992. View Abstract

Lansdorp PM, Sutherland HJ, & Eaves CJ. Selective expression of CD45 isoforms on functional subpopulations of CD34+ hemopoietic cells from human bone marrow. J Exp Med 172 (1): 363-6, 1990. View Abstract

Sutherland HJ, Eaves CJ, Eaves AC, Dragowska W, & Lansdorp PM. Characterization and partial purification of human marrow cells capable of initiating long-term hematopoiesis in vitro. Blood 74 (5): 1563-70, 1989. View Abstract

van den Engh G, Trask B, Lansdorp P, & Gray J. Improved resolution of flow cytometric measurements of Hoechst- and chromomycin-A3-stained human chromosomes after addition of citrate and sulfite. Cytometry 9 (3): 266-70, 1988. View Abstract

Lansdorp PM, Aalberse RC, Bos R, Schutter WG, & Van Bruggen EF. Cyclic tetramolecular complexes of monoclonal antibodies: a new type of cross-linking reagent. Eur J Immunol 16 (6): 679-83, 1986. View Abstract

 

TFL pic

Shannon Valdez

Bsc, PMP
Research Projects Coordinator
Ester

Dr Ester Falconer

PhD
Postdoctoral Fellow
Vancouver, BC --  26 November 2013 - Mark Hills  (Photo: BC Cancer Agency)

Dr Mark Hills

PhD
Postdoctoral Fellow
Ashley Sanders

Ashley Sanders

Graduate Student
Yu_Amy

Amy Yu

BA, PMP
Research Projects Manager