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Selection aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress

Posted October 19, 2017 | Publications

Mathew V, Tam AS, Milbury KL, Hofmann AK, Hughes CS, Morin GB, Loewen CJR & Stirling PC. Selection aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress.  J Cell Biol [Epub ahead of print].

Abstract

Upon genotoxic stress, dynamic relocalization events control DNA repair as well as alterations of the transcriptome and proteome, enabling stress recovery. How these events may influence one another is only partly known. Beginning with a cytological screen of genome stability proteins, we find that the splicing factor Hsh155 disassembles from its partners and localizes to both intranuclear and cytoplasmic protein quality control (PQC) aggregates under alkylation stress. Aggregate sequestration of Hsh155 occurs at nuclear and then cytoplasmic sites in a manner that is regulated by molecular chaperones and requires TORC1 activity signaling through the Sfp1 transcription factor. This dynamic behavior is associated with intron retention in ribosomal protein gene transcripts, a decrease in splicing efficiency, and more rapid recovery from stress. Collectively, our analyses suggest a model in which some proteins evicted from chromatin and undergoing transcriptional remodeling during stress are targeted to PQC sites to influence gene expression changes and facilitate stress recovery.